DNA BARCODING OF JOJOBA (Simmondsia chinensis) PLANTS CULTIVATED IN EGYPT USING rbcL GENE
Abstract
Precise identification of jojoba plant species using DNA barcode is very important because of the economic value of its seed oil. In this study, we selected 16 jojoba trees (clones) including one male and 15 female trees cultivated in the same farm to conduct this experiment. DNA samples were extracted from leaf tissues and subjected to PCR amplification using specific primers for rbcL gene fragment. The amplification was successful for all tested samples producing the same fragment with a size about 550 bp. Sequence analysis was performed for the purified products and consequently subjected to GenBank database analysis. The data analysis produced by BLASTn database revealed that the similarity search was from 98-100% with rbcL gene region form available DNA sequence in GenBank. Multiple sequence alignment was performed after confirmation of the DNA sequence. The alignment clearly indicated the high similarity between all DNA sequences for the tested samples. Furthermore, a phylogenetic tree was produced from the alignment process which revealed the close relationship between the tested samples. The tree can be divided into two main branches one containing samples 1-13 and 16 while the other branch contains samples 14 and 15. These results indicate that the rbcL region is a good option for molecular identification of jojoba species. Finally, the obtained sequence data were submitted to GenBank to provide the accession numbers for the nucleotide sequences.
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