CLONING AND CHARACTERIZATION OF CUCUMBER MOSAIC VIRUS COAT PROTEIN GENE FROM INFECTED BANANA PLANTS IN EGYPT
Abstract
Cucumber mosaic virus was detected in banana plants showing typical symptoms of CMV infection using DAS-ELISA. Coat protein cDNA was ampli- fied, by RT-PCR using specific primers, and cloned into pGEM®-T Easy Vector, then transformed into E. coli Top 10 cells. The complete sequence of coat protein gene from CMV-Egy strain has been de- termined and compared to other known CMV-CP sequences. The alignment of the amino acid sequences revealed high ho- mology (98-100%) between CMV-Egy and different CMV strains. Phylogenetic analysis also revealed that CMV-Egy is closely related with CMV-Fny isolated from USA and Sny isolated from Israel.References
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