CLONING AND CHARACTERIZATION OF CUCUMBER MOSAIC VIRUS COAT PROTEIN GENE FROM INFECTED BANANA PLANTS IN EGYPT

Authors

  • HANAN A. NOUR EL-DIN Department of Microbial Molecular Biology, AGERI, ARC, 9 Gamaa St. Giza, 12619, Egypt
  • R. SALEM Department of Plant Molecular Biology, AGERI, ARC, 9 Gamaa St. Giza, 12619, Egypt
  • K. S. ABDALLA Department of Plant Molecular Biology, AGERI, ARC, 9 Gamaa St. Giza, 12619, Egypt

Abstract

Cucumber mosaic virus was detected in banana plants showing typical symptoms of CMV infection using DAS-ELISA. Coat protein cDNA was ampli- fied, by RT-PCR using specific primers, and cloned into pGEM®-T Easy Vector, then transformed into E. coli Top 10 cells. The complete sequence of coat protein gene from CMV-Egy strain has been de- termined and compared to other known CMV-CP sequences. The alignment of the amino acid sequences revealed high ho- mology (98-100%) between CMV-Egy and different CMV strains. Phylogenetic analysis also revealed that CMV-Egy is closely related with CMV-Fny isolated from USA and Sny isolated from Israel.

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2016-01-12

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