ANALYSIS OF DIFFERENTIAL GENE EXPRESSION IN RESPONSE TO DROUGHT STRESS IN WHEAT (Triticum aestivum L.) USING cDNA-SCoT
Abstract
A cDNA- start codon-targeted polymorphism (cDNA-SCoT) marker was employed to isolate and characterize differentially expressed sequence tags (ESTs) in response to drought stress in wheat (Triticum aestivum L.) at seedling stage, cultivar Giza 186. Three weeks old wheat seedlings were subjected to drought stress, the seedlings sampled at different time points (1 h and 6 h) after exposed to drought stress with three biological replicas for each treatment, and untreated seedlings were used as control. An array of 17 differentially expressed EST fragments in drought-stressed plants comparing to control was obtained, and characterized using the bioinformatics analysis. A cDNA-SCoT marker showed a variation in different gene expressions profiling between treated and untreated seedlings under drought stress.
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