DEVELOPMENT OF AFLP, ISSR AND RAPD MARKERS FOR HIGH YIELD-RELATED TRAITS IN JOJOBA

Authors

  • NAHLA A. AWAD Fruit Breeding Dept., Horticultural Research Institute (HRI), Agriculture Research Center (ARC).
  • S. D. IBRAHIM Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC).
  • S. S. ADAWY Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC).
  • M. A. OMAR Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC).

Abstract

Jojoba is an economic oil crop as its seeds store liquid wax (40-60% of dry weight). Due to wide variations in yield, selection of superior female plants is a big challenge to increase the yield in future populations. In this study, fifty female jojoba strains were evaluated for 13 traits representing morphology, seed characteristics and yield. Six strains representing the extremes for yield and seed weight traits were selected for molecular analysis. The selected strains were characterized using 8 AFLP, 16 ISSR and 30 RAPD primers/primer combinations. For yield, the AFLP, ISSR and RAPD produced 531, 138 and 325 total scorable bands with percentage polymorphism of 28.0, 35.5 and 35.6, respectively. While for seed weight, they generated 524, 135 and 317 total scorable bands with percentage polymorphism of 27.0, 31.1 and 34.0, respectively. The phylogenetic analysis for yield, successfully grouped the superior strains in one cluster while for seed weight, the superior strains were grouped in another cluster except for ISSR dendrogram. These results represent the first study combining different molecular markers, agronomical and morphological evaluation of 13 traits aimed at developing unique positive and negative markers which can be used to identifying superior jojoba strains in early stages of development.

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Published

2019-04-13

Issue

Vol. 47 No. 2 (2018)

Section

Articles