MAPPING OF QTLs CONTROLLING RESISTANCE TO LEAF STRIPE (Pyrenophora graminea) IN EGYPTIAN BARLEY

Authors

  • S. S. ADAWY Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, 12619-Egypt
  • ROBA M. ISMAIL Plant Genetic Transformation Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, 12619-Egypt
  • M. M. SAKR Plant Biotechnology Dept., National Research Center, Egypt

Abstract

Leaf stripe in barley, caused by Pyrenophora graminea, is an important seed-borne disease in organically grown as well as in conventionally grown Nordic and Mediterranean barley districts. In the present work, a QTL map for F2 barley and 7 LSLs) distributed on 8 linkage groups and spanning 2099.4 cM of the barley genome. The size of linkage groups varied from 117.8 cM for LG2 (2H) to 482.9 cM for LG6 (6H) with an average length of 262.4 cM. Based on the used anchor SSR markers, only four linkage groups were assigned to chromosomes, where LG2, LG5, LG6 and LG7 were assigned to chromosomes 2H, 5H, 6H and 7H, respectively. Single point analysis was used to identify genomic regions controlling the leaf stripe severity and plant height traits. A total of 40 QTL were identified for the disease severity (DS) and plant height (PH) traits (20 QTL for disease severity and 20 QTL for plant height). This work represents the first genetic linkage map for barley population derived from an intraspecific cross between At20 and At29 showing chromosomal regions associated with disease severity and plant height traits in barley.

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