DETERMINATION OF GENOTОXIC EFFECTS OF SOME FOOD ADDITIVES ON SOME HUMAN CANCER CELLS BY FLOW CYTOMETRY ANALYSIS

Authors

  • SHIMAA E. RASHAD National Research Center, Giza
  • F. M. ABDEL-TAWAB Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • EMAN M. FAHMY Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • A. G. ATTALLAH National Research Center, Giza
  • EKRAM S. AHMED National Research Center, Giza
  • A. A. HAGGRAN National Research Center, Giza

Abstract

Toxicogenomics is the study of the relationships between the structure and activity of the genome and the adverse biological effects of exogenous agents. Different kinds of additives are widely applied in food industry. Yeast cells were grown with the food additives, and culture growth. This study aimed to select and genetically determine the possible genotоxic effects of three food additives (MSG, SB and Saffron) on human cell lines. Flow cytometric analysis demonstrated that treatment of human hepatocellular carcinoma cells (Caco-3) cells with food additives increased G2/M phase cell cycle arrest. The quantitative real time-PCR was used to measure the mRNA levels of p53, Bax, and Bcl-2 genes. The data showed that food additives changed transcriptional levels of these related genes. The mRNA expression of p53 and Bax were up-regulated, but, the transcription of Bcl2 was significantly down-regulated compared to the control. Protein-protein interaction maps provided a valuable framework for a better understanding of the functional organization of the proteome. These data indicated that food additives decreased cell viability in malignant and non-malignant cells as well as confirmed the occurrence of their cytоtoxic effects. It is evident from the aforementioned discussion that the studied food additives could inflect some serious health hazards if they are added haphazardly in foods and preveradges. Therefore, strict control of their use in accordance with the special regulations set by the Food and Drug Administration (FDA) and World Health Organization (WHO) to insure their biosafety for human consumption.

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Published

2019-04-13

Issue

Vol. 47 No. 2 (2018)

Section

Articles

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