DETERMINATION OF GENOTОXIC EFFECTS OF SOME FOOD ADDITIVES ON SOME HUMAN CANCER CELLS BY FLOW CYTOMETRY ANALYSIS

Authors

  • SHIMAA E. RASHAD National Research Center, Giza
  • F. M. ABDEL-TAWAB Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • EMAN M. FAHMY Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • A. G. ATTALLAH National Research Center, Giza
  • EKRAM S. AHMED National Research Center, Giza
  • A. A. HAGGRAN National Research Center, Giza

Abstract

Toxicogenomics is the study of the relationships between the structure and activity of the genome and the adverse biological effects of exogenous agents. Different kinds of additives are widely applied in food industry. Yeast cells were grown with the food additives, and culture growth. This study aimed to select and genetically determine the possible genotоxic effects of three food additives (MSG, SB and Saffron) on human cell lines. Flow cytometric analysis demonstrated that treatment of human hepatocellular carcinoma cells (Caco-3) cells with food additives increased G2/M phase cell cycle arrest. The quantitative real time-PCR was used to measure the mRNA levels of p53, Bax, and Bcl-2 genes. The data showed that food additives changed transcriptional levels of these related genes. The mRNA expression of p53 and Bax were up-regulated, but, the transcription of Bcl2 was significantly down-regulated compared to the control. Protein-protein interaction maps provided a valuable framework for a better understanding of the functional organization of the proteome. These data indicated that food additives decreased cell viability in malignant and non-malignant cells as well as confirmed the occurrence of their cytоtoxic effects. It is evident from the aforementioned discussion that the studied food additives could inflect some serious health hazards if they are added haphazardly in foods and preveradges. Therefore, strict control of their use in accordance with the special regulations set by the Food and Drug Administration (FDA) and World Health Organization (WHO) to insure their biosafety for human consumption.

References

Al-Senosy, N. K., A. A. Awad, R. A. A. Younis and F. M. Abdel-Tawab (2018). The phthalate dbp-induced cytotoxicity and apoptosis via gene expression of P53, Bcl2 and Bax in tumor cell lines. Egypt. J. Genet. Cytol., 47: 45-56.

Ataseven, N., D. Yuzbasioglu, A. C. Keskin and F. Unal (2016). Genotoxicity of monosodium glutamate. Food Chem. Toxicol., 91: 8-18.

Cann, M., D. Barrett, A. Cooper, A. Crumpler, D. Dalen, L. Grimshaw,

K. Kitchin and E. K. Lok (2007). Food additives and hyperactive behaviour in 3-year-old and 8/9-year-old children in the communit a randomised, double-blinded, placebo-controlled trial. Journal of Experimental Psychology: General, 370: 1000-1016.

Chung, T., L. Shih-Chao, S. Jui-Hsin, C. Yu-Kuo, L. Chi-Chien and C. Hong-Lin (2017). Sinularin induces DNA damage, G2/M phase arrest, and apoptosis in human hepa-tocellular carcinoma cells. Complementary and Alternative Medicine, 17: 62.

El-Magd, M. A., H. E. Abbas, A. M. El-kattawy and A. Mokhbatly (2013). Novel polymorphisms of the IGF1R gene and their association with average daily gain in Egyptian buffalo (Bubalus bubalis). Domestic Animal Endocrinology, 45: 105-110.

Eweka, A. O., P. S. Igbigbi and R. E. Uchey (2011). Histochemical studies of the effects of monosodium glutamate on the liver of adult Wistar rats. Ann. Med. Health Sci. Res., 1: 21-29.

Farmobi, E. O. and O. O. Onyemia (2006). Monosodium glutamate-induced oxidative damage and genotoxicity in the rat: Modulatory role of vitamin C, vitamin E and quercetin. Hum. Exp. Toxicol., 25: 251-259.

Leu, J. I., P. Dumont, M. Hafey, M. E. Murphy and D. L. George (2004). Mitochondrial p53 activates Bak and causes disruption of a BakMcl1 complex. Nat. Cell Biol., 6: 443-450.

Livak, K. J. and T. D. Schmittgen (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods, 25: 402-408.

Milajerdi, A., K. Djafarian and B. Hosseini (2016). The toxicity of saffron (Crocus sativus L.) and its constituents against normal and cancer cells. Journal of Nutrition and Intermediary Metabolism, 3: 23-32.

Moreno, M. C. (2005). Effect of glaucoma on the retinal glutamate/glutamine cycle activity. The FASEB Journal. https://doi.org/10.1096/fj.04-3313fje

Osfor, M., S. A. El-Desouky and N. A. El-Leithy (1997). Effect of dietary intake of monosodium glutamate on some nutritional and biochemical traits in albino rats. Eg. J. Comp. Path. Clin. Path., 10: 131-139.

Patel, D. and R. Ramani (2017). In vitro determination of genotoxic effects of sodium benzoate preservative on human peripheral blood lymphocytes. Inter. J. Res. Biosciences, 6: 20-26.

Pavlović, V., S. Cekić, G. Kocić, D. Sokolović and V. Živković (2007). Effect of monosodium glutamate on apoptosis and Bcl-2/Bax protein level in rat thymocyte culture. Physiological Research, 56: 619-626.

Polager, S. and D. Ginsberg (2009). p53 and E2f: Partners in life and death. Nat. Rev. Cancer, 9: 738-748.

Sleiman, R. J. and B. W. Stewart (2000). Early caspase activation in leukemic cells subject to etoposide induced G2-M arrest: evidence of commitment to apoptosis rather than mitotic cell death. Clinical Cancer Research, 6: 3756-3765.

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2019-04-13

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