SOMACLONAL VARIATION FROM MATURE EMBRYO EX- PLANTS OF SOME EGYPTIAN BARLEY GENOTYPES

Authors

  • SHAIMAA E. RASHAD Microbial Genetics Dept., National Research Center
  • F. M. ABDEL-TAWAB Genetics Dept., Faculty of Agriculture, Ain Shams University
  • EMAN M. FAHMY Genetics Dept., Faculty of Agriculture, Ain Shams University
  • M. M SAKR Plant Biotechnology Dept., National Research Center

Abstract

Somaclonal variation is used to describe the occurrence of genetic variants derived from in vitro procedures; it is also called culture-induced variation. Many plant biotechnology applications use plant tissue culture as a tool, and the unpredictable nature of these variations represents a serious problem, especially for the commercial applications of such technology. Such variations in micro propagated material, such as genetic and epigenetic modifications, need to be characterized. Biochemical genetic analysis (protein and isozymes) and molecular genetic analysis (RAPD-PCR) were applied to study somaclonal variations in three barley genotypes (El-Kasr, G130 and G126). In conclusion, this study demonstrated the establishment of an influence of different media composition on the embryogenic response and regeneration of the different barley genotypes. Biochemical and molecular genetic analysis of protein, isozymes and RAPD-PCR proved to be efficient tools for detecting the occurrence of somaclonal variation in the three barley genotypes El-kasr, G126 and G130.

References

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2020-08-30 — Updated on 2020-12-15

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Vol. 49 No. 1 (2020)

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