ANTI-CANCER EFFECTS OF Zygophyllum album AND Suaeda palaestina EXTRACTS ON HUMAN LIVER CANCER CELL LINES
Abstract
The present study aims to the assessment of anticancer activity of the extracts of the two medicinal plants Zygophyllum album and Suaeda palaestina., in a previous study, human liver cancer cell lines (HepG2) showed the cytotoxicity of the two plants by MTT assay. The half-maximal inhibitory concentration (IC50) observed in the (HepG2) was 27.74 μg/ml in Z. album, while IC50 of S. palaestina was 30.76 μg/ml. According to IC50 of both plants, the flow cytometric analysis was investigated and indicated that the treatment of HepG2 cells with Z. album and S. palaestina led to substantial elevation of G2/M phase cell cycle arrest. The real time-PCR was used to measure the mRNA levels of cyclin B1 and cyclin dependent kinase (CDK1) genes. Both genes showed down-regulation of expression levels in HepG2 with Z. album and S. palaestina compared to the untreated cells. The apoptotic mechanism activated by both plant extracts resulted in up-regulations of p53 and Caspase 3 and the down-regulation of Bcl-2 on mRNA levels on HepG2 after treatment with both extracts. In conclusion, these results suggest that Z. album and S. palaestina could be considered as candidate species being a good natural source of anticancer agents.References
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