ASSESSMENT OF ANTICANCEROGENIC EFFECT OF Tamarix nilotica ON HUMAN LUNG CANCER CELL LINE

Authors

  • F. M. ABDEL-TAWAB Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • SHAIMAA A. SHEBL Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • NEIMA K. ALSENOSY Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • HALIMA S. ABDOU Department of Cell Biology, National Research Centre, Dokki, Giza

Abstract

Samples of Tamarix nilotica were collected from Wadi Gharandal, South Sinai, Egypt in March 2015 by Desert Research Center (DRC).Plants was extracted by Ethyl acetate. Possibility of anticancer activity of crude extract was assessed against human lung carcinoma (A549) cell lines to identify the best extraction solvent. Results showed the extract of plant was significantly active against the human lung carcinoma (A549). Cytotoxic activity onA549 andWi38 cell lines by Neutral red assay indicated that T. nilotica ethyl acetate crude extract did not affect normal cell lines (Wi38). In contrast, it induced cyto-toxicity on cancer cell lines (A549) as shown by a more intense decrease in cell viability on human cancer cell lines. Flow cytometry-basedcell cycle distribution showed that the percentage of A549 cells arrest at G2/M phase was markedly increased after treatment which confirmed the cytotoxic effect on cancer cells So, these results indicated that T. nilotica has antiproliferative effect. Analyses of apoptotic cells by (AO/EtBr) immunofluorescence double staining indicated that the crude extract showed an increase of apoptosis. Regarding the control, the nuclear region of viable cells was uniformly green in color, whereas, early apoptotic cells were orange with bright dots corresponding to nuclear chromatin fragmentation. Late apoptotic cells showed intensive red nucleus. Cells treated with T. nilotica extract exhibited characteristic changes of apoptosis e.g. fragmentation and formation of apoptotic bodies,The expression levels of the apoptosis-related genes p53, Bcl-2 and BAX in (A549) were assessed by quantitative real time PCR to determine the ability of T. nilotica to induce apoptosis in cancer cells (A549), compared with untreated group. Our results indicated that the expression levels of p53 and Bax were increased, whereas that of Bcl-2 was decreased. The results suggest that T. nilotica could be considered good natural source of anti-cancer agents.

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Published

2019-08-04

Issue

Vol. 48 No. 1 (2019)

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