ISOLATION AND IDENTIFICATION OF KEY GENES CONTROL- LING SALT TOLERANCE IN GREY MANGROVE (Avicennia mari- na) AL-NABQ PROTECTORATE, EGYPT
Abstract
DNA barcoding by internal transcribed spacer (ITS) was obtained. DNA sample was amplified from mangrove by the ITS1 and ITS4 primers), the PCR product exhibited a single band of the target size (545bp). BLASTn search results of Avicennia marina; 18S ribosomal RNA geneas a partial sequence; complete sequence of internal transcribed spacer 1 (ITS1), 5.8S ribosomal RNA gene and internal transcribed spacer 2 (ITS2)); and 26S ribosomal RNA gene as a partial sequence, confirmed our results. Molecular characterization of the 3'-UTR-structured region of each of the five gene confirmed the high homologies of amCat genes (99%), amFer (99%) amSOD (98%) and UBC2 (100%). It was evident that the variation in the 3'-UTR region is very limited because it is harboring conserved regulatory region that controls the efficiency and half-life time of mRNA and thus it is reflected on the gene expression. These genes have important roles in controlling salt, oxidative and osmotic stresses in the Egyptian gray mangrove plant under the red sea conditions within the Nabq protectorate in South Sinai.References
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