GENETIC TRANSFORMATION OF EGYPTIAN WHEAT WITH 1DX5 HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNIT GENE
Abstract
Variation in the composition of the high-molecular-weight glutenin subunits (HMW-GS) of wheat is associated with large differences in the bread-making properties. The primary aim of this work was to produce transgenic Egyptian wheat cultivar (Giza 164) with HMW-GS gene (1DX5) to improve bread-making quality. Immature embryo-derived calli were cotransformed with pK-DX5 and pACH25 plasmids containing gus and bar genes using biolistic bombardment. Stable integration and expression of marker genes were detected in seven plants. However, the expression of 1DX5 gene was confirmed in five plants using SDS-PAGE analysis.References
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