TRANSGENIC EGYPTIAN WHEAT (Triticum aestivum L.) WITH Arabidopsis NPR1 GENE via BIOLISTIC BOMBARDMENT

Authors

  • A. H. FAHMY Agriculture Genetic Engineering Research Institute, Agricultural Research Center, Egypt
  • K. EL MANGOURY Agriculture Genetic Engineering Research Institute, Agricultural Research Center, Egypt
  • WALAA ABOU EL-WAFA Plant Science Department, Faculty of Science, Ain Shams University, Egypt
  • HODA M. S. BARAKAT Plant Science Department, Faculty of Science, Ain Shams University, Egypt
  • SOHEIR EL-SAEED EL-KHODARY Plant Science Department, Faculty of Science, Ain Shams University, Egypt
  • J. LI Department of Plant Pathology, Kansas State University, Manhattan, KS, USA
  • S. MUTHUKRISHNAN Department of Biochemistry, Kansas State University, Manhattan, KS, USA

Abstract

AtNPR1 gene plays a crucial role in biotic resistance in plants. Immature embryo derived calli of wheat cv. Giza 164 were co-transformed with the plasmid pAHC25 containing bar and gus genes and the plasmid pJS406 containing AtNPR1 gene via Biolistic bombardment. The produced calli were subcultured on bialaphos containing medium (CIMB). Surviving calli were then regenerated on selection medium (MSRB) to produce green regenerated shoots, which were then transferred onto rooting/selection medium (FMSB). Regenerated rooted plantlets were transferred to soil pots in the control growth chamber for acclimatization. Only 48 plants succeeded in acclimatization and reached seed setting in biocontainment greenhouse. Transgene integration was confirmed by PCR and dot-blot analyses. The overall transformation frequency was 1.9%.

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Published

2017-08-06

Issue

Vol. 46 No. 1 (2017)

Section

Articles