ISOLATION AND CHARACTERIZATION OF A GEMINIVIRUS ISOLATED FROM SUGAR BEET PLANTS IN EGYPT
Abstract
Sugar beet is one of the main sources for sugar manufacturing in Egypt, however its cultivation and growing can be severely hampered due to viral infection. In this study beet plants infected with geminivirus-like symptoms (VLS), such as leaf curling, leaf rolling, and stunting plants growth, were collected from different several locations and examined for the presence of geminivirus through indirect DAS ELISA using two antiserums, namely C4 antiserum specific for tomato yellow leaf curl virus (TYLCV), which gave negative results, and TYLCV polyclonal antiserum which is common for any white-bean gave negative result. Electron microscope examination showed virus-like particles scattered in the cells of positive plant. Additionally, significant cytopathological effects due to viral infection were observed in the nuclei and many other organelles. Furthermore, the typical geminivirus amplicons from rolling circle amplification (RCA) combined with restriction fragment digestion amplification, which were detected in those infected host plants. Our results demonstrated that the isolated virus from infected beet plants belongs to the whitefly-transmitted geminiviruses (WTGs). Based on transmission patterns, virus host range, and sequence analysis of C1 region of viral genome, we suggest that the virus presented in this study can be considered as a new isolate of Tomato yellow leaf curl virus (TYLCV).References
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