THE PHTHALATE DBP-INDUCED CYTOTOXICITY AND APOP-TOSIS VIA GENE EXPRESSION OF p53, Bcl2 AND Bax IN TUMOR CELL LINES
Abstract
The components of phthalate are utilized as softeners and plasticizers in a wide range of plastic materials. There has been a high concern of potential health risks to humans. DBP is commonly used as plasticizer in food containers, furniture, toys, cosmetics, cellulose plastics, latex adhesives and dye solvents. The present study focused on the cytotoxic activity of the DBP and its pos-sible underlying mechanisms were also investigated. The results showed that phathalate di-n-butyl phthalate (DBP) induced profound cytotoxicity in cancer cells, i. e. Human hepatocellular carcino-ma (HepG2) (IC50 = 43.77 μg/ml), against human lung cancer cell line (A549) (IC50 = 106.3 μg/ml). Moreover, DBP exhibited cytotoxic activity on nor-mal cell lines; the IC50 of DBP is 254 μg/ml and 445.3 μg/ml on THLE2 and Wi38, respectively. Flow cytometric analysis demonstrated that treatment of HepG2 cells with DBP increased G2/M phase cell cycle arrest. The quantitative real time-PCR was used to measure the mRNA levels of p53, Bax, and Bcl-2 genes. The data showed that DBP changed transcriptional levels of these apoptosis-related genes. The mRNA ex-pression of p53 and Bax were up-regulated, but, the transcription of Bcl2 was significantly down-regulated com-pared to the control.References
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