NUTRITIONAL AND BIOLOGICAL EVALUATION OF TRANSGENIC POTATO LINES RESISTANT TO POTATO TUBER MOTH
Abstract
The present study was designed to estimate the substantial equivalence of genetically modified (GM) potato Spunta lines (SpG2, SpG3 and Sp6A-3) with the cry1Ic1 gene compared to conventional non-transgenic potato Spunta cultivar (control group) through rat feeding experiments and through the determination of nutritional composition such as: protein, ash, fiber, fat, starch, total carbohydrates, amino acids, fatty acids, micro and macroelements and vitamins B1, B2 and C. Short-term feeding study was conducted for nine days using experimental rats to estimate the digestibility parameters: True Digestibility, (T.D.), Biological Value (B.V.) and Net Protein Utilization (N.P.U.) as well as the behavior of rats under investigation. Rats in each group (SpG2, SpG3 and Sp6A-3 and control groups) grew normally without marked differences in appearance, behavior or in mortality rate. No marked differences were observed in the T.D., B.V. and N.P.U. in all groups. All tested chemical parameters showed no significant differences between the GM samples and the control. From these results, it can be concluded that the GM potato Spunta lines (SpG2, SpG3 and Sp6A-3) are equivalent to the non-GM potato Spunta cultivar. The results for allergenicity testing did not support the idea that the cry1IcI gene may cause any allergic reaction to the tested animalsReferences
Anderson, D. M., J. M. Bell and G. I. Christson (1977). Can. J. Anim. Sci., 58: 87-96.
AOAC (1995). Washington DC, USA.
AOAC (2000). Association of Official Analytical Chemists, Official Method of Analysis, 25th edition, Benjamin Franklin Station.
AOAC (2000). Association of Official Analytical Chemists, Official Method of Analysis, 17th edition, 969.3 and 991.39: Fatty acids in oil and fat. Chapter 41, p 19-20.
Chapman, H. D. and P. F. Pratt (1961). Methods of ananlysis for soil, plants and waters. Agric. Publ. Univ. of California, Riverside. Ching_Fang H., T. Tsung-Ya and P. Tzu-
Ming (2005). Use of the duplex taqman PCR system for the detection of shiga-like toxin producing E.coli O157:H7. Journal of Clinical Microbiology, June: 2668-2673.
Codex A. C. (2003). Alinorm 03/34: Joint FAO/WHO Food Standard Programme, Codex Alimentarius Commission, Twenty-Fifth Session, Rome, Italy 30 June-5 July, Appendix III, Guideline for the conduct of food safety assessment of foods derived from recombinant- DNA plants and Appendix IV, Annex on the assessment of possible allergenicity, p. 47-60.
Cottenic, A., M. Verloo, L. K. Kens, G. Velghe and R. Camerlynck (1982)."Cemical Analysis of Plants and Soils". Laboratory of Analytical and Agrochemistry State University, Ghent-Belgium.
Douches, D. S., W. Pett, F. Santos, J. Coombs, E. Grafius, W. L. Li, E. A. Metry, T. Nasr EL-Din and M. Madkour (2004). Field and storage testing Bt potatoes for resistance to potato tuberworm (Lepidoptera: Gelichiidae). Journal of Economic Entomology, 97: 1425-1431.
Eggum, B. O (1973). Study of certain factors influencing protein utilization in rats and pigs. PhD thesis, Institute of Animal Science, Royal Veterinary and Agriculture Univ., Copenhagen, Denmark.
Food and Agriculture Organization of the United Nations (FAO) (1996). Biotechnology and food safety. FAO food and nutrition paper 61. Report of a joint FAO/WHO consultation. Rome, Italy.
Food and Agriculture Organization of the United Nations FAO/WHO. (2000). Safety Aspects of Genetically Modified Foods of Plant Origin. Report of a Joint FAO/WHO.
Fujimoto H., K. Itoh, M. Yamamoto, J. Kyozuka and K. Shimamoto (1993). Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis. Bio/Technology, 11: 1151-1155.
Gomez, K. A. (1984). Statistical Procedures for Agricultural Research, John Wiley and Sons, New York, pp. 139.
Habiba, A. R., S. El-Samahy, S. Awny, S. El-Desouky (2000). Food Science, 22: 5-13.
Hashimoto, W., K. Momma, T. Katsube, Y. Ohkawa, T. Ishige, M. Kito, S. Utsumi and K. Murata (1999). Food Agriculture, 79: 1607-1612.
Koziel, M. G., G. L. Beland, C. Bowman, N. B. Carozzi, R. Crenshaw, L. Crossland, J. Dawson and S. V. Evola (1993). Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio/Technology, 11: 194-200.
Marianna, S. (2000). The concept of substantial equivalence in safety assessment of foods derived from genitically modified organisms. AgBiotechNet, 2: April ABN 044.
Mohammed, A. A., D. S. Douches, W. Pett, E. Grafius, J. Coombs, Li. W. Liswidowati and M. A. Madkour (2000). Evaluation of potato tuber moth (Lepidoptera: Gelechiidae) resistance in tubers of Bt-cry5 transgenic potato lines. J. Econ. Entomol, 93: 472-476.
Nayak, P., D. Basu, S. Das, A. Basu, D. Ghosh, N. A. Ramakrishnan, M. Ghosh and S. K. Sen (1997). Transgenic Elite indica rice plants expressing Cry1Ac delta endotoxin of Bacillus thuringiensis are resistant against yellow stem borer (Scirpophaga incertulas). Proc. Natl. Acad. Sci. USA, 94: 2111- 2116.
Newell, C. A., R. Rozman, M. A. Hinchee, E. C. Lawson, L. Haley, P. Sanders, W. Kaniewski, N. E. Tumer, R. B. Horsch and R. T. Fraley (1991). Agrobacterium mediated transformation of (Solanum tuberosum L.) cv. 'Russet Burbank'. Plant Cell Reports, 10: 30- 34.
NMKL (1996): Nordic Committee on Food Analysis; Determination of Vitamins B1 and B2 by HPLC in food and feed. Method number 189.2.
NMKL (1996): Nordic Committee on Food Analysis; Determination of Vitamin C by HPLC in food and feed. Method number 113.2.
Official Journal of the European Communities (1998). Determination of amino acids.
Organization for Economic Cooperation and Development, OECD (1996). Food safety and evaluation; Paris, France.
Organization for Economic Cooperation and Development, OECD (1997). Report of the OECD workshop on the toxicological and nutritional testing of novel food; Paris, France.
Organization for Economic Cooperation and Development, OECD. (2000). Report of the OECD Task Force for the Safety of Novel Foods and Feeds. Okinawa. Japan.
Ross, H. (1986) Potato breeding: problems and perspectives. Journal of plant breeding Supplement 13: 1-132.
Tavazza, R., M. Tavazza, R. J. Ordos, G. Ancora and E. Benvenuto (1988). Genetic transformation of potato (Solanum tuberosum L.). An efficient method to obtain transgenic plants. Plant Science, 59: 175-181.
Vaeck, M., A. Reynaerts, H. Hofte, S. Jansens, M. D. Beukeleer, C. Dean, M. Zebeau, M. V. Montagu and J. Leemans (1987). Transgenic plants protected from insect attack. Nature (London), 238: 33-37.
World Health Organization, WHO (1995). Strategies for assessing the safety of foods produced by biotechnology. Report of the joint FAO/WHO consultation. World Health Organization, Geneva.