EVALUATION OF GENETICALLY MODIFIED POTATO LINES RESISTANT TO POTATO VIRUS Y
Abstract
Nine putative transformed potato lines resistant to potato virus Y (PVY) were successfully micropropagated in vitro using nodal cutting technique on MS salts medium. Adaptation and acclimatization of the transgenic potato lines were carried out in the Bio-containment greenhouse facilities at AGERI. The percentage of survival was 74 to 100%. The plantlets were challenged artificially with a strain of potato virus Y. Five highly resistance lines to PVY infection were determined according to ELISA and Dot-blot analysis. Evaluation of transgenic and non-transgenic potato lines under field experiment was conducted at Assiut University. Such evaluation included vegetative growth characteristics (plant height, leaves and stems number per plant), yield and yield components (tubers number, size, weight and total yield/plant). Challenging with virus strain was also conducted mechanically in the field and the resistance was determined visually and by ELISA test. Field plantation confirmed data obtained at greenhouse experiments. It can be concluded that five of the putative transformed lines exhibited high resistance to PVY under field conditions.References
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