TRANSFORMATION OF CHITINASE GENE TO RESIST EARLY BLIGHT DISEASE IN SOME POTATO VIRUS RESISTANT LINES
Abstract
Potato (Solanum tuberosum L.) an agro-economically important food crop in the world. It is sensitive to many fungal pathogens including Alternaria solani, the causal agent of early blight disease. In the present study, pRI 201-AN binary vector, used in potato transformation, containing the NPT-II selectable marker gene in plant, containing the chitinase gene. De-siree cultivar, PVY5 and PVY15 lines (resistant to potato virus Y) were trans-formed with the pRI construct via the Ag-robacterium delivery system. Chitinase gene was transformed into leaves of pota-to gene types. Transformed leaves were incubated on MS medium with a 5 mg/L of 2-4,D. After that, leaves transferred to regeneration media which contained MS medium with a 1 mg/L of IAA, a 1 mg/L of BA, a 10 mg/L of GA3, a 1 mg/L of cefatoxine (200 mg/ml) and a 1 mg/L of kanamycine (25 mg/ml). After 10 weeks of transformation, the regeneration results of Desiree cultivar, PVY5 and PVY15 lines were 96.6%, 71.4% and 73.5%, re-spectively. Their expression at the tran-scriptional level was confirmed by poly-merase chain reaction (PCR).References
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