NUTRITIONAL AND BIOLOGICAL EVALUATION OF TRANSGENIC POTATO LINES RESISTANT TO POTATO TUBER MOTH

Authors

  • E. A. METRY Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt
  • GIHAN M. EL MOGHAZY Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • M. S. MASOUD Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • M. H. YOUSSEF Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • T. A. OMAR Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • T. YEHYA Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • S. A. MAHFOUZ Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • A. M. SHOKRY Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt
  • M. F. EMARA Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • A. S. HAMZA Regional Center for Food and Feed (RCFF), ARC, Giza, Egypt
  • T. M. NASR EL-DIN Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt
  • HANAYIA. A. EL-ITRIBY Agricultural Genetic Engineering Research Institute (AGERI), ARC, Giza, Egypt

Abstract

The present study was designed to estimate the substantial equivalence of genetically modified (GM) potato Spunta lines (SpG2, SpG3 and Sp6A-3) with the cry1Ic1 gene compared to conventional non-transgenic potato Spunta cultivar (control group) through rat feeding experiments and through the determination of nutritional composition such as: protein, ash, fiber, fat, starch, total carbohydrates, amino acids, fatty acids, micro and macroelements and vitamins B1, B2 and C. Short-term feeding study was conducted for nine days using experimental rats to estimate the digestibility parameters: True Digestibility, (T.D.), Biological Value (B.V.) and Net Protein Utilization (N.P.U.) as well as the behavior of rats under investigation. Rats in each group (SpG2, SpG3 and Sp6A-3 and control groups) grew normally without marked differences in appearance, behavior or in mortality rate. No marked differences were observed in the T.D., B.V. and N.P.U. in all groups. All tested chemical parameters showed no significant differences between the GM samples and the control. From these results, it can be concluded that the GM potato Spunta lines (SpG2, SpG3 and Sp6A-3) are equivalent to the non-GM potato Spunta cultivar. The results for allergenicity testing did not support the idea that the cry1IcI gene may cause any allergic reaction to the tested animals

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2016-01-13

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