ANTIMUTAGENIC EFFECTS OF CHITOSAN AGAINST THE CY- TOLOGICAL AND BIOCHEMICAL CHANGES INDUCED BY AFLATOXINS IN RATS
Abstract
The purpose of this study was to investigate the possible antimutagenic effects of chitosan against genotoxic effects induced by aflatoxin in different mammalian cell systems. The chromosomal abnormalities in bone marrow cells as well as liver total proteins and blood serum isozymes in male rats were assayed after 45 and 90 days of treatments. The results indicated that aflatoxin significantly (p<0.01) increased chromosomal abnormalities in bone marrow cells after both of 45 and 90 days of treatments, while the effect of chitosan was significant (p<0.05) only after 90 days of treatment. Treating rats with aflatoxins contaminated diet in addition to chitosan resulted in a significant decrease in chromosomal aberrations compared to the single treatments for both experimental periods. The results of electrophoretic separation of total protein and isozymes revealed the presence of some bands with higher intensity in aflatoxin group, while it was less for the combined treatments compared to the corresponding bands of the control group. The results suggested that chitosan has an antimutagenic effect against aflatoxin.References
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