MOLECULAR CHARACTERIZATION OF DROUGHT TOLER-ANCE IN NINE EGYPTIAN RICE GENOTYPES USING RAPD, SCoT AND SSR MARKERS
Abstract
Nine Egyptian rice (Oryza sativa L.) genotypes were assessed for DNA polymorphism using three different types of molecular markers (RAPD, SCoT and SSR). RAPD primers produced 68.70% of polymorphism and an average PIC value of 0.24. SCoT primers generated 86.54% of polymorphism and 0.28 average value of PIC. All tested SSR markers yielded amplified products and generated 104 alleles (average 6.5 alleles/marker) with PIC values ranged from 0.20 to 0.49 per marker. RM301 SSR marker produced specific alleles only in the four drought sensitive rice varieties that could readily distinguished the sensitive genotypes from the others. While, RM20A and RM302 SSR markers produced one positive unique marker in the moderate (Sakha
102) and drought tolerant (Giza 178) varieties, respectively. These markers may be usefully exploited for molecular breeding of rice for drought tolerance. On the other hand, clustering analysis using UPGMA method classified the nine rice genotypes into three groups using RAPD and SCoT markers and four groups using SSR marker. The results of principal coordinate analysis (PCoA) were closely related with those of the clustering analysis. These results could be used by breeders to develop drought tolerant rice genotypes and new breeding protocols for rice improvement.
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