EVALUATION OF THE PROTECTIVE EFFECT OF JOJOBA NAT-URAL PRODUCTS ON HEPATOTOXICITY OF DIETHYL NI-TROSAMINE IN RATS

Authors

  • M. MOAWAD Department of Surgical Pathology, National Cancer Institute, Cairo University
  • A. E. HEGAZY Plant Biotechnology Dept., Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University
  • GHADA M. NASR Department of Molecular Diagnostics, Genetic Engineering and Biotechnology Research Institute, Sadat City University
  • S. A. SAKR Zoology Department, Faculty of Science, Menoufia University, Shebin El-Kom
  • A. K. HANDA Department of Horticulture and Landscape Architecture, Purdue University, West Lafayette
  • M. I. NASR Molecular Biology Dept., Genetic Engineering and Biotechnology Research Institute (GEBRI), Sadat City University

Abstract

The current study was designed to achieve the following objectives: Studying the effect of diethyl nitrosamine (DEN) on rats’ liver; and the effect of jojoba oil on the liver enzymes and DNA content. Ad-ministration of DEN induced a significant increase in levels of serum ALT and AST. Administration of jojoba oil alone or with DEN caused significant decrease in serum levels of ALT and AST, compared with the control group through the experi-mental period. The level of lipid per oxi-dation (MDA) levels increased in DEN treated group. On the other hand animals treated with jojoba and DEN showed a decrease in lipid per oxidation. Compared with the DNA content showed that jojoba oil provoked a massive accumulation of cells in the three cell phases (G0/G1, S and G2/M), this led to the appearance of a diploid DNA content peak in the cell cycle experiments which is characterized to apoptotic cell population. The histological results revealed that jojoba oil improved the alterations caused by DEN.

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Published

2018-09-06

Issue

Vol. 47 No. 1 (2018)

Section

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