MOLECULAR DIAGNOSIS OF THE AVEN GENE AS A PROGNOS-TIC FACTOR IN BREAST CANCER PATIENTS
Abstract
In the present study, we investigated the possible role of Aven gene and its expression at the transcription level detected by RT-PCR technique as a prognostic factor in breast cancer to confirm the prognosis assessed by the immunohisto-chemical analysis of Vegf and Ki67 genes, and ER, PR status.
The present study was performed on 40 diagnosed female breast cancer patients and 10 cases benign breast tissues. The fresh tumor tissues were divided into two fragments for histological, immuno-histological analysis and for the RT-PCR analysis.
In this study, we evaluated a conventional RT-PCR assay for Aven gene as a detection marker of 40 IDC patients. The findings were analyzed to assess the prognostic significance of Aven gene expression-detection in the breast tissues in association with the other known prognostic factors. Statistically significant association was found between positive Aven gene expression in the IDC and well established prognostic parameters; namely, Ki67, Vegf and hormonal status (P value <0.0001).
In breast cancer, Aven gene is considered responsible for circulating tumor tissues and its expression might constitute a biologically active subset of breast cancer patients with high tumor burden and bad prognosis. As the expression of Aven gene is easily determined by conventional RT-PCR, it may be useful marker to predict prognosis of breast cancer.
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