GENETIC DIVERSITY ASSESSMENT AMONG SIX RABBIT BREEDS USING RAPD AND SRAP MARKERS
Abstract
Rabbits consider one of the important animal farm species as a source for healthy and nutrient meat in Egypt and studying the genetic diversity among rabbit breeds is very important key for breeders to develop new breeds with superior economic traits. In the present study nine RAPD and seven combinations of SRAP primers were used to analyze the genetic diversity among six rabbit breeds namely Bouscat, Gabali, California, Chinchilla, Flemish, and New zealand. Also, the efficiency of the markers and the primers sets were investigated. The used primers of RAPD and SRAP generated 102 and 67 bands, respectively. Some of RAPD primers (OPA-08, OPC-07, OPE-07, OPM-07 and OPK-17) and SRAP primers combinations (Em3-Me2, Em2-Me5, Em10-Me5 and Em10-Me10) were more informativeness and showed higher discriminative power or generated specific bands for some rabbit breeds; these primers could be useful in further studies. The cluster analysis of combined data generated form RAPD and SRAP banding profile patterns revealed similarity coefficient values ranged from 0.739 between Bouscat and Chinchilla to 0.842 between Bouscat and Gabali. Additionally, from genetic similarity matrix and dendrogram analysis it appears that Bouscat, Gabali and California are more closely related and differ from Chinchilla, Flemish and New Zealand which are more closely related.References
Abdou Basha, H., W. S. H. Abd El-Naby and H. S. Mohammed (2016). Ge-netic diversity and phylogenetic relationship among three Duck. Adv. Anim. Vet. Sci., 4: 462-467.
Abouzaid, E., E. N. El-Sayed, E. A. Mo-hamed and M Youssef (2016). Mo-lecular analysis of drought toler-ance in guava based on In vitro PEG evaluation. Tropical Plant Biol., 9: 73-81.
Barwar, A., M. L. Sangwan, S. Kumar and S. Ahlawat (2008). Genetic diversi-ty between Murrah and Bhadawari breeds of Indian buffalo using RAPD-PCR. Ind. J. Biotech., 7: 491-495.
Bargish, T. A. and F. Rahmani (2016). SRAP markers based genetic anal-ysis of Silene species. Journal of Tropical Biology and Conserva-tion, 13: 57-70.
Brem, G., P. Hartl, U. Besenfelder, E. Wolf, N. Zinovieva and R. Pfaller (1994). Expression of synthetic cDNA sequences encoding human insulin-like growth factor-1 (IGF-1) in the mammary gland of trans-genic rabbits. Gene, 149: 351-355.
Ceron, A. and F. Angel (2001). Genetic diversity in sugarcane hybrids in Colombia measured using molecu-lar markers. Proc. Int. Soc. Sugar-cane Technol. of North America, 24: 626.
Coulibaly, S., U. Besenfelder, I. Miller, N. Zinovieva, C. Lassnig, T. Kotler, J. L. Jameson, M. Gemeiner, M. Mul-ler and G. Brem (2002). Expression and characterization of functional recombinant bovine follicle-stimulating hormone (boFSHalpha/beta) produced in the milk of transgenic rabbits. Mol. Reprod. Dev., 63: 300-308.
El Fadly, G., I. Khatab, M. Rehan and A. Kalboush (2016). Genetic diversity in Egyptian populations of Tilapia species using RAPD and SRAP markers. J. Biodiv. and Env. Sci., 8: 231-243.
El-Bayomi, K. M., A. Awad and A. A. Saleh (2013). Genetic diversity and phylogenetic relationship among some rabbit breeds using random amplified polymorphic DNA markers. Life Science, 10: 1449-1457.
El-Sabrout, K. and S. A. Aggag (2014). Phylogenetic relationships among different lines of rabbits in Egypt. Global J. Res. Rev., 1: 112-116.
El-Sabrout, K. and A. El-Raffa (2015). Molecular characterization of Al-exandria rabbit line using DNA markers. Rabbit Gen., 5: 1-5
Fan, J., S. Kitajima, T. Watanabe, J. Xu, J. Zhang, E. Liu and Y. E. Chen (2015). Rabbit models for the study of human atherosclerosis: from pathophysiological mechanisms to translational medicine. Pharmacol. Ther., 146: 104-119.
Galal, O. A., M. Rehan and R. E. Abd El-Karim (2013). Analysis of genetic diversity within and among four rabbit genotypes using biochemical and molecular genetic markers. Af-rican J. Biotech., 12: 2830-2839.
Gwala, P. E., N. W. Kunene, C. C. Bezuidenhout and B. S. Mavule (2015). Genetic and phenotypic variation among four Nguni sheep breeds using random amplified polymorphic DNA (RAPD) and morphological features. Trop. Anim. Health. Prod., 47: 1313-1319.
Heikrujam, M., J. Kumar and V. Agrawal (2015). Genetic diversity analysis among male and female Jojoba genotypes employing gene targeted molecular markers, start codon tar-geted (SCoT) polymorphism and CAAT box-derived polymorphism (CBDP) markers. Meta Gene, 5: 90-97.
Jaccard, P. (1908). Nouvelles recherches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat., 44: 223-270.
Kantanen, J., J. Vilkki, K. Elo and A. Mäki-Tanila (1995). Random am-plified polymorphic DNA in cattle and sheep: application for detecting genetic variation. Anim. Genet., 26: 315-320.
Keliang, R., L. Yanping, H. Dongchang, W. Xinsheng, Z. Ping, L. Quanzhong, Z. Lijun, Z. Shenghua and C. Liang (2008). Study of rela-tionship of Rex rabbit RAPD marker and reproductive perfor-mance. 9th World Rabbit Congress - June 10 -13, Verona - Italy.
Khaled, A. G. A., G. A. R. El-Sherbeny, T. M. El-Sheikh and A. A. Katana (2018). Identification of RAPD molecular markers linked to pheno-typic characteristics in Rabbits breeds. Journal of Sohag Agriscience, 1: 36-49.
Lai, A. C. K., W. L. Wu, S. Lau, Y. Guan and H. Chen (2012). Two-dimensional antigenic dendrogram and phylogenetic tree of avian in-fluenza virus H5N1. FEMS Immunol. Med. Microbiol., 64: 205-211.
Li, G. and C. F. Quiros (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet., 103: 455-461.
Li, L., W. Shen, L. Min, H. Dong, Y. Sun and Q. Pan (2006). Human lactoferrin transgenic rabbits pro-duced efficiently using dimethylsulfoxide-sperm-mediated gene transfer. Reprod. Fertil. Dev., 18: 689-695.
Li. Q. Y., S. J. Dong, W. Y. Zhang, R. Q. Lin, C. R. Wang, D. X. Qian, Z. R. Lun, H. Q. Song and X. Q. Zhu (2009). Sequence-related amplified polymorphism, an effective molec-ular approach for studying genetic variation in Fasciola spp. of human and animal health significance. Electrophoresis, 30: 403-409.
Mandal, R., S. Nag, J. Tarafdar and S. Mitra (2016). A comparison of ef-ficiency parameters of SSR mark-ers and genetic diversity analysis in Amorphophallus paeoniifolius (Dennst.) Nicolson. Braz. Arch. Biol. Technol., 59: 1-7.
Moghaieb, R. E. A, A. A. Abdelhadi, H. A. El-Sadawy, N. A. T. Allam, B. A. Baiome, M. H. Soliman (2017). Molecular identification and genet-ic diversity among Photorhabdus and Xenorhabdus isolates. 3 Bio-tech, 7: 6.
Mollah, M. B. R., F. B. Islam, M. S. Is-lam, M. A. Ali and M. S. Alam (2009). Analysis of genetic diversi-ty in Bangladeshi chicken using RAPD markers. Biotechnology, 8: 462-467.
Mufti, M. M. R., M. P. Mostari, G. K. Deb, K. Nahar and K. S. Huque (2009). Genetic diversity of red Chittagong cattle using randomly amplified polymorphic DNA markers. American J. Anim. Vet. Sci., 4: 1-5.
Osman, M. M., S. A. Hemeda, A. A. I. Hassanin and A. H. El Aswad (2010). Molecular genetic evalua-tion of six rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR. Suez Canal Vet. Med. J., 15: 1-10.
Peng, X. (2012). Transgenic rabbit models for studying human cardiovascular diseases. Comp. Med., 62: 472-479.
Rangoju, P. K., S. Kumar, A. P. Kolte, R. Gulyani and V. K. Singh (2007). Assessment of genetic variability among rabbit breeds by random amplified polymorphic DNA (RAPD)-PCR. World Rabbit Sci., 15: 3-8.
Revaldaves, E., E. Renesto and J. R. Gold (2016). Genetic variation of Prochilodus lineatus (Valenci-ennes, 1836) from Paraná, Baía, Miranda, and Corumbá rivers, Bra-zil. Genet. Mol. Res., 15: 1-11.
Saengprajak, J. and P. Saensouk (2012). Genetic diversity and species iden-tification of cultivar species in subtribe Cucumerinae (Cucurbitaceae) using RAPD and SCAR markers. American Journal of Plant Sciences, 3: 1092-1097.
Saini, M., S. Singh, Z. Hussain and K. Sikka (2010). RAPD analysis in mungbean [Vigna radiata (L.) Wilczek.] II: A comparison of effi-ciency parameters of RAPD pri-mers. Indian Journal of Biotech-nology, 9: 276-282.
Schmitt, G. and P. Barrow (2017). Regu-latory approaches to non-clinical reproductive toxicity testing of an-ti-cancer drugs. Anticancer Agents Med. Chem., 17: 1171-1183.
Shams, S. S., S. Z. Vahed, F. Soltanzad, V. Kafil, A. Barzegari, S. Atashpaz and J. Barar (2011). Highly effec-tive DNA extraction method from fresh, frozen, dried and clotted blood samples. BioImpacts, 1: 183-187.
Song, H. Q., X. H. Mo, G. H. Zhao, J. Li, F. C. Zou, W. Liu, X. Y. Wu, R. Q. Lin, Y. B. Weng and X. Q. Zhu (2011). Electrophoretic detection of genetic variability among Schistosoma japonicum isolates by sequence-related amplified poly-morphism. Electrophoresis, 32: 1364-1370.
Sun, S. J., W. Gao, S. Q. Lin, J. Zhu, B. G. Xie and Z. B. Lin (2006). Anal-ysis of genetic diversity in Ganoderma population with a nov-el molecular marker SRAP. Appl. Microbiol. Biotechnol., 72: 537-543.
Williams, J. G., A. R.Kubelik, K. J. Livak, J. A. Rafalski and S. V. Tingey (1990). DNA polymorphisms am-plified by arbitrary primers are use-ful as genetic markers. Nucleic Ac-ids Research, 18: 6531-6535.
Yu, M. B. Ma, X. Luo, L. Zheng, X. Xu and Z. Yang (2008). Molecular di-versity of Auricularia polytricha revealed by inter-simple sequence repeat and sequence-related ampli-fied polymorphism markers. Curr. Microbiol., 56: 240-245.
Zhu, Z. H., H. Y. Li, Y. Qin and R. X. Wang (2014). Genetic diversity and population structure in Harpadon nehereus based on se-quence-related amplified polymor-phism markers. Genet. Mol. Res., 13: 5974-5981.
