DETERMINATION OF THE RELATIONSHIPS BETWEEN Pasteurella multocida ISOLATED FROM DIFFERENT FARM ANI-MALS AND THEIR HOST RANGE CONTACTS IN EGYPT USING BIOCHEMICAL AND MOLECULAR TECHNIQUES

Authors

  • G. M. HASSAN Genetics Department, Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt
  • Z. A. EL-FEKY Genetics Department, Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt
  • E. A. EISSA Genetics Department, Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt
  • A. T. AHMED Genetics Department, Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt

Abstract

The aim of the present study was to identify and compare the variations among clinical of P. multocida isolates originating from different hosts, sheep (n = 4), buffalo (n = 4) and cattle (n = 4). These isolates were obtained from various locations in the Fayoum governorate, Egypt and they were analyzed by morphological, API kit and RAPD PCR at molecular level to check the distribution of Pneumonia and hemorrhagic septicemia in different farm animals. The chosen isolates exhibited luxuriant growth on blood agar with translucent grayish or yellowish green colonies. However, they showed no growth on MacConkey and no hemolysis of blood; also isolates were positive for oxidase, catalase and H2S production, negative of urease and can ferment glucose, fructose and glucose but can't ferment lactose. Antibiotic sensitivity showed that isolates were sensitive to tetracycline, clindamycin and erythromycin but resistant to ampicillin, trimethoprim/sulfamethoxazole and flucloxacillin. To determine the genetic diversity nine random primers OPA-01  OPA09 were used to which generate different profiles. The distinct RAPD profiles of p. multocida strains showed specificity with regard to the host and the disease induced.

References

Armstrong, J. S., A. J. Gibbs, R. Peakall and G. Weiler (1994). The RAPD Distance package, Ver. 1.03. Research School of Biology Science Australia National University. Canberra, Australia.

Balakrishnan, G. and R. Parimal (2012). Isolation, identification and antibiogram of Pasteurella multocida of avian origin. Tamilnadu J. Veterinary & Animal Sciences, 8: 199-202.

Bauer, A. W., W. M. Kirby, J. C. Sherris and M. Truck (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology, 45: 493-496.

Chaslus-Dancla, E., M. C.Lesage-Descauses, S. Leroy, J. L. Martel and J. P. Lafont (1996). Validation of random amplified polymorphic DNA assays by ribotyping as tools for epidemiological surveys of Pasteurella multocida. Veterinary of Microbiology, 52: 91-102.

CLSI (Clinical and Laboratory Standard Institute). (2009). Procedure Manual for Laboratory Practice. 3rd Edition. 1400, Wayne, Pennsylvania USA, 1887-1898.

Coates, S. R. and K. H. Hoopes (1980). Sensitivities of E. coli isolated from bovine and porcine enteric infection to antimicrobial antibiotics. American Journal of Veterinary Research, 41: 1882-1883.

Cowan, S. T. and K. L. Steel (2004). Manual for Identification of Medical Bacteria. 2nd Edition, Cambridge University Press, Cambridge. p. 28-106.

Dewani, P. (2000). Bacteriological studies on mastitis in ewes and goats. M. Sc. (Hons) Thesis, Department of Microbiology. Sindh Agriculture. University Tandojam. India.

Excoffier, L., P. E. Smouse and J. M. Quattro (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 131: 479-491.

Gunawardana, G. A., K. M. Townsend and A. J. Frost (2000). Molecular characterization of avian Pasteurella multocida isolates from Australia and Vietnam by REP-PCR and PFGE. Veterinary Microbiology, 72: 97-109.

Hawari, A. D., D. S. Hassawi and M. Sweiss (2008). Isolation and identification of Manheimia haemolytica and Pasteurella multocida in sheep and goats using biochemical tests and random amplified polymorphic DNA (RAPD) analysis. Journal of Biology Science, 8: 1251-1254.

Hird, D. W., T. E. Carptenter, K. P. Snipes, D. C. Hirsh and R. H. Mccapes (1991). Case control study of fowl cholera in meat turkeys in California. American Journal of Veterinary Research, 52: 212- 216.

Jabbari, A. R. and M. Esmaelizadeh (2005). Molecular typing of avian Pasteurella multocida isolates by PCR-RFLP of oomph gene. Iranian Journal of Biotechnology, 3: 99-103.

Jared, D., L. Taylor, W. F. Robert, S. D. Mady, W. L. Terry and W. Anthony (2010). Comparison of genotypic and phenotypic characterization methods for Pasteurella multocida isolates from fatal cases of bovine respiratory disease. Journal of Veterinary Diagnosis Investigation, 22: 366-375.

Miflin, J. K. and P. J. Blackall (2001). Development of a 23S rRNA-based PCR assay for the identification of Pasteurella multocida. Letters in Applied Microbiology, 33: 216-221.

Munir, R., D. Shahwar, U. Farooq, I. Nawaz, I. Shahzad and A. Khanum (2007). Outer membrane protein profiling of Pasteurella multocida. Pakistan Veterinary Journal, 27: 1-4.

OIE (Office International Des Epizootics) (2004). Fowl Cholera. Terrestrial Manual, 9: 524-530.

Owen, R. J. (1989). Chromosomal DNA fingerprintinga new method of species and strains identification/application to microbial pathogens. Journal of Medical Microbiology, 30: 89-99.

Ozbey, G., G. A. Kilic, H. B. Ertas and A. Muz (2004). Random amplified polymorphic DNA (RAPD) analysis of Pasteurella multocida and Manheimia haemolytica strains isolated from cattle, sheep and goats. Veterinary Medical Czech, 49: 65-69.

Rhoades, K. R. and R. B. Rimler (1991). Avian Pasteurellosis, in diseases of poultry. 8th ed. Iowa State University Press aims Iowa. p. 141- 156.

Sabiel, Y. A., M. T. Musa and V. Z. Ann (2012). Identification of Mannheimia haemolytica and Pasteurella multocida by polymerase chain reaction and random amplification of polymorphic DNA. Sudan Journal of Veterinary Research, 27: 55-59.

Sikora, S., S. Redzepovic, I. Pejic and V. Kozumplik (1997). Genetic diversity of Bradyrhizobium japonicum field population revealed by RAPD fingerprinting. Journal Applied Microbiology, 82: 527-531.

Tang, X., Z. Zhao, J. Hu, B. Wu, X. Cai, O. He and H. Chen (2009). Isolation antimicrobial resistance and virulence genes of Pasteurella multocida strains from swins in China. Journal of Clinical Microbiology, 47: 951-958.

Tefera, J. S. (2002). Differentiation of Pasteurella multocida and Pasteurella haemolytica using Entero Rapid-24 Test Kit. Veterinary Medical Czech, 47: 99-103.

Townsend, K. M., H. J. Dawkins and J. M. Papadimitriou (1997). REP-PCR analysis of Pasteurella multocida isolates that cause hemorrhagic septicemia. Research Veterinary Science, 63: 151-155.

Venkatesan, P. S., P. Wmanohar, K. S. Palaniswamp, M. Parthiban and C. Andrew (2006). Random amplified polymorphic DNA-polymerase chain reaction analysis on animal Pasteurellosis. Indian Journal of Animal Science, 76: 571-574.

Zomuankima, V., T. K. Dutta, P. Roychoudhury, B. Jubeda and C. Rajesh (2014). Isolation, identification, characterization and antibiogram of Pasteurella multocida isolated from pigs in Mizoram with special reference to progressive atrophic rhinitis. Journal of Veterinary World, 2231-2916.

Downloads

Published

2016-01-23

Issue

Vol. 44 No. 2 (2015)

Section

Articles

Most read articles by the same author(s)