CHITINASE AND CELLULASE GENES SEQUENCING FOR SOME EGYPTIAN Trichoderma species ISOLATED FROM RHIZOSPHERE AND ASSAY OF THEIR ACTIVITY
Abstract
Hydrolytic enzyme producing Trichoderma species have long been rec-ognized as an agent for controlling plant diseases caused by various phytopathogenic fungi. A study was in-tended to identify highly chitinase and cellulose isolated Trichoderma producer from the rhizosphere. Chitinase gene iso-lated from Trichoderma strains FUE3, FUE5, FUE6, FUE9, FUE15 and I18 had one fragment with length of 1039 bp, en-coding 344 amino acids. Cellulase gene isolated from Trichoderma strais FUE15 had one fragment with length of 204 bp, encoding 68 amino acids. The results pre-sented showed that Trichoderma strains I18, FUE9, FUE6, FUE3, FUE5 and FUE15 exhibited significant activities of chitinase compare to control. The high potent cellulase producer was detected by FUE5 being 0.075 mg/ml whereas, the lowest figure produced by FUE15 being 0.024 mg/ml.References
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