MOLECULAR GENETIC FINGERPRINTING FOR NEW SELECTED EGYPTIAN SHEEP STRAINS IN RELATION TO OSSIMI BREED
Abstract
Two strains of El-Adely sheep and Ossimi sheep breed were characterized using biochemical techniques. Protein banding pattern using SDS and native gel electrophoresis were carried out to identify the biochemical genetic fingerprinting of each El-Adely sheep strains and Ossimi breed. The genetic homogeneity percentage based on Native-protein within each of Ossimi, El-Adely (HORS) and El-Adely (HMYS) were 53%, 37% and 55%, respectively. Similarity value based on SDS-PAGE, Native-PAGE and RAPD-PCR analysis was the highest between the two strains of El-Adely sheep (HOR and HMY) strains, while it was the lowest between Ossimi breed and El-Adely sheep HOR strain. Also, similarity value was low between El-Adely sheep HMYstrain and Ossimi breed. The dendrogram was constructed among the studied sheep groups based on SDS-PAGE, Native-PAGE and RAPD analyses showed that Ossimi breed was distantly related from both the two strains of El-Adely sheep (HOR and HMY strains).
The present study revealed that SDS-PAGE, Native-PAGE and RAPD-PCR techniques could be strongly recommended as useful tools for detecting fingerprint, determination of polymorphism and establishing the genetic relationships among different sheep genotypes.
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