MOLECULAR GENETIC FINGERPRINTING FOR NEW SELECTED EGYPTIAN SHEEP STRAINS IN RELATION TO OSSIMI BREED

Authors

  • I. M. K. KAYALI Dept. of Genetics, Fac. of Agric., Ain Shams University
  • M. A. RASHED Dept. of Genetics, Fac. of Agric., Ain Shams University
  • M. R. ANOUS Dept. of Animal Production, Fac. of Agric., Ain Shams University

Abstract

Two strains of El-Adely sheep and Ossimi sheep breed were characterized using biochemical techniques. Protein banding pattern using SDS and native gel electrophoresis were carried out to identify the biochemical genetic fingerprinting of each El-Adely sheep strains and Ossimi breed. The genetic homogeneity percentage based on Native-protein within each of Ossimi, El-Adely (HORS) and El-Adely (HMYS) were 53%, 37% and 55%, respectively. Similarity value based on SDS-PAGE, Native-PAGE and RAPD-PCR analysis was the highest between the two strains of El-Adely sheep (HOR and HMY) strains, while it was the lowest between Ossimi breed and El-Adely sheep HOR strain. Also, similarity value was low between El-Adely sheep HMYstrain and Ossimi breed. The dendrogram was constructed among the studied sheep groups based on SDS-PAGE, Native-PAGE and RAPD analyses showed that Ossimi breed was distantly related from both the two strains of El-Adely sheep (HOR and HMY strains).
The present study revealed that SDS-PAGE, Native-PAGE and RAPD-PCR techniques could be strongly recommended as useful tools for detecting fingerprint, determination of polymorphism and establishing the genetic relationships among different sheep genotypes.

References

Ali, B. A. (2003). Genetic similarity among four breds of sheep in Egypt detected by random amplified polymorphic DNA markers. African Journal of Biotechnology, 7: 194-197.

Appa Rao, K. B. C., K. V. Bhat and S. M. Totey (1996). Detection of species specific genetic markers in farm animals through random amplified polymorphic DNA (RAPD). Genetic Analysis: Biomolecular Engineering, 13: 135-138.

Awad, N. S. (2005). Molecular genetic studies on some Egyptian sheep breeds. PhD. Thesis, Department of Genetics, Faculty of Agriculture, Ain Shamas University, Egypt.

Baker, C. M. A. and C. Manwell (1991). Population genetic molecular marker and gene conservation of bovine breeds. Cattle Genetic Resource (ed. By G. C. Hichman). 221-304.

Bardakci, F. and D. O. F. Skibinski (1994). Application of RAPD technique in tilapia fish: species and subspecies identification. Heredity, 73: 117-123.

Bollag, M. and J. Edestein (1994). Protein Methods. Fourth Edition. Wilye-Liss, Inc. 605 Thrid Avenue, New York.

Elmaci, C. and S. Asal (2000). Blood biochemical polymorphism in Angora Goat Breed. Egyptian J. of Anim. Prod., 37: 31-36.

El-Seoudy, A. A., M. Nermin Abdel Gawad, Asmaa M. Abu Shady and A. Z. E. Abdelsalam (2005). Biochemical and molecular genetic characterization of some Egyptian goat breeds. Egypt. J. Genet. Cytol., 34: 63-79.

Hames, B. D. and D. Rickwood (1981). Gel electrophoresis pf proteins: a particle approach. IRL Press Washington, D.C.

Hillis, D. M., B. K. Mable, A. Larson, S. K. Davis and E. A. Zimmer (1996). Nucleic acids IV: Sequencing and cloing, In: D. M. Hillis, C. Moritz, B. Mable (Eds.), Molecular systematics 2nd ed., p. 342-343. Sunderland, Massachusetts: Sinauer Associates, Inc.

Houle, D. (1989). Generics, 123: 789-801. Lynch, M. (1990). The similarity index and DNA fingerprinting. Mol. Bio. Evol., 7: 478-484.

Mervild, A. (2007). Middle East Regional Veterinary Information Livestock Data. Ministry of Agriculture-Economic Affairs, Secretary for Agriculture economic 2007.

Mourad, M. (2008). El-Adely: A new sheep breed in Egypt. Animal Science Reporter, Vol 2, Issue 3.

Sneath, P. H. A. and R. R. Sokal (1973). Numerical Taxonomy. W. H. Freeman, San Francisco.

Tapio, M., I. Grigaliunaite, L. E. Holm, S. Jeppson, J. Kantanen, I. Miceikiene, I. Olsaker, H. Vinalass and E. Eytnorsdottir (2002). Mitochondrial differentiation in Northern European sheep. Proc. of the 7th World Congress on Genetics Applied to Livestock Production (WCGALP).

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2016-01-12

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