CYTOCHROME OXIDASE SUBUNIT I GENE BASED IDENTIFICA- TION OF THE COMMON EGYPTIAN TILAPIINE SPECIES

Authors

  • Y. G. FITEHA Animal Department, Collage of Women, Ain Shams University, Cairo
  • M. MAGDY Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo
  • HAGER T. ELHIFNAWY Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo
  • AMIRA EL- KEREDY Genetics Department, Faculty of Agriculture, Tanta University, Tanta
  • R. A. M. ALI Animal Department, Collage of Women, Ain Shams University, Cairo
  • M. A. RASHED Genetics Department, Faculty of Agriculture, Tanta University, Tanta

Abstract

DNA barcoding has become a massively applied tool for accurate and rapid identification of various taxa using COI gene. The current study aimed to identify different species of Egyptian tilapiine using cytochrome oxidase subunit I (COI) gene. Total seven specimens were sampled, representing three species Oreochromis niloticus, Tilapia zillii and Sarotherodon galilaeus. DNA was extracted, PCR was performed, a conventional assay using gel electrophoresis, purified the amplicons, sequenced and analyzed. This study has validated the efficacy of COI gene for identifying fish species. The Egyptian tilapiine identity was confirmed and stated that this marker is suitable for its molecular identification. Additionally, it was successful to identify closely related species and determine the genetic relationship among them.

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Published

2020-08-30

Issue

Vol. 49 No. 1 (2020)

Section

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