MICRO RNA192 EVALUATION AS EARLY DIABETIC RETINOPATHY DIAGNOSTIC BIOMARKER IN Egyptian PATIENTS WITH TYPE 2 diabetes mellitus
Abstract
Short non-coding RNAs known as microRNAs have been associated with different disorder types, like diabetes mellitus (DM) and its complications such diabetic retinopathy and nephropathy. In order to ealy diagnose diabetic retinopathy (DR), the study purpose was to assess the expression level of miRNA 192 in type 2 diabetes patients and explore its association with these problems. The participants in the current study were 30 healthy non-diabetic people and 70 type 2 diabetes patients who were categorized into two main groups according to the time from the onset of DM (age and sex- matched). Diabetic retinopathy is one of the most common consequences of diabetes. The complete set of data was collected, including sociodemographic and laboratory data. RT-PCR assay was used to determine the levels of miRNA192 expression in whole blood. All diabetic groups, particularly diabetic patients with retinopathy, had mean expression levels of miRNA 192 that were considerably greater than those of healthy subjects. The expression levels of miRNA 192, blood glucose and HbA1c, were significantly positively correlated in the group with diabetic retinopathy. Mir-192 had a sensitivity of 83.3% in diabetic retinopathy and specificity of 100 % at the specified cutoff. According to the findings, up-regulated of miRNA 192 in type 2 diabetes is correlated to the prevalence of diabetic retinopathy. Warning indications of diabetes complications could be miRNA 192.
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