IDENTIFICATION OF FRAGRANCE GENE fgr IN THE EGYPTIAN JASMINE RICE (EJR) AND ITS CROSSES WITH THREE CHINESE CYTOPLASMIC MALE STERILE LINES
Abstract
Aroma Egyptian Jasmine rice (EJR) is one of the most important types, It is one of the high quality rice varieties all over the world, have the fragrance gene fgr. Three cytoplasmic male sterile lines, and EJR were hybridized. The three cytoplasmic male sterility Chinese lines were: Hexi41B, Yimi15B and D297 and their crosses were Hexi41B x E.Jasmine (HJR), Yimi15B x E. Jasmine (YJR) and D297 x E. Jasmine (DJR). The results showed that the male sterile lines outperform the Egyptian Jasmine rice in most of morphological trail such as; 1000 grain weight, plant height, number of panicles plant, and grain yield for plant traits. For identification of fgr gene primers were used in PCR reaction, the fgr gene was identified in the Egyptian Jasmine rice (EJR) and its hybrid lines. The gene was also digested using three different restriction enzymes (RE) such as; EcoR1, BamH1 and Hind ІІІ. By estimating the total amount of protein in the grains, the result noticed that it reached 6.91 in the Egyptian Jasmine rice (EJR) and about 6.91(grain with cover), while it reached the highest amount in DJR hybrid as crosse between the Egyptian Jasmin rice (EJR) and male sterility lines leads to fgr gene transfer enhancement and increase of protein content in rice grains which reached up to 8.3 in D297 x Egyptian Jasmine rice hybrid (EJR). The main target of this study was to determine the fragrance gene (fgr) from EJR in cytoplasmic male strile (CMS) rice after crosses between them and the EJR, that resulting in changes of charachterstics after transferring the gene to these CMS lines.
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