MORPHOLOGICAL AND MOLECULAR GENETICS CHARAC- TERIZATION OF HOLOPARASITIC PLANT, Cistanche phelypea L. IN SIWA OASIS, EGYPT

Authors

  • H. E. ELWAKIL Department of Agricultural Botany, Faculty of agriculture-Saba Basha, Alexandria Univ., Egypt
  • N. R. ABDELSALAM Department of Agricultural Botany, Faculty of agriculture-Saba Basha, Alexandria Univ., Egypt
  • REHAM M. ABD EL-AZEEM Dept. of Environ. Biotechnology, Institute of Genet. Eng. and Biotechnology, Univ. of Minufiya
  • A. A. HEMEIDA Dept. of Bioinformatics, Institute of Genetic Engineering and Biotechnology, Univ. of Minufiya
  • NERMEEN Y. ABASS Department of Agricultural Botany, Faculty of agriculture-Saba Basha, Alexandria Univ., Egypt
  • A. NASSAR Plant Protection Dept., Faculty of Agricultural, Damnhour Univ.

Abstract

Cistanche phelypea (Fam. Orbanchaceae) is a parasite plant on the root of the host plant. The main natural hosts are the woody species of family chenopodiaceae such as Arthrocnemum macrostachyum and family Zygophyllacea such as Zygophyllum album. It is an obligate parasite which totally depends on its host for water, minerals and organic nutrients. Many species belonging to the same family have the same phenomenon such as orbanche crenata Forsk which attacks legume plants, specially, Vicia faba. Cistanche phelypea L., grows in Egypt in low density but scattered on a large area of the country. It can be recognized in the Nile region including Faiyum, the Oases of the western desert including Siwa, the Red Sea coastal strip, and the entire Sinai Peninsula including the coastal of the Mediterranean. It grows in sandy and alluvial soil, edges of cultivated fields as a parasite on plant roots. In this study the species was surveyed and photographed in Siwa Oasis and in different obliterated Oasis around Siwa Depression. For the first time in Egypt, morphological, chemical study, RAPD molecular markers and biochemical genetic analysis of Peroxidase isozymes were carried out in order to identify the genetical and morphological description of the species including the identification of genetic variation in the studied populations.

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2016-01-12

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