DIVERSITY AND EVOLUTION OF CYP MITOCHONDRIAL GENE IN NILE TILAPIA (Oreochromis niloticus L.)

Authors

  • M. A. RASHED Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
  • AMIRA EL-KERADY
  • M. MAGDY Genetics Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Abstract

The cytochrome P450 (CYP) superfamily of heme-containing enzymes catalyzes Phase I biotransformation of endogenous and xenobiotic compounds, including fatty acids, steroids, drugs, and environmental contaminants. Common variations (polymorphisms) in cytochrome P450 genes can affect the function of the enzymes. The effects of polymorphisms are most prominently seen in the breakdown of medications. In fish, members of the CYP2 and CYP3A families play a major role in the metabolism of xenobiotics and endogenous compounds. The current study aimed to isolate and compare cytochrome P450 3A40 gene from Oreochromis niloticus (Nile tilapia) to understand its diversity and evolution in comparison with fresh water fish species available in the GenBank database. Total length of 1300 bp was obtained and its polymorphism with similar samples from O. niloticus in the GenBank was determined. Site no. 41 T>A changed the amino acid from Phenylalanine (F) to Tyrosine (Y), while sites no. 43 and 54 caused no effect (silent mutations), and site no. 74 A>G changes the amino acid from Glutamic Acid (E) to Glycine (G), in which the two later mutations formed a different protein isoform in its conformational structure. Phylogenetic analysis reflected a clear divergence of fresh water families (Cichlidae and Poecilidae) from other families, while fixed and shared mutations between families were found. Phylogenetic analysis provided strong support for the identity of the majority of CYPs in fresh water fishes. In the current study, conservative regions among the studied families were found.

References

Barber, D. S., A. J. McNally, N. Garcia- Reyero and N. D. Denslow (2007). Exposure to p,p′-DDE or dieldrin during the reproductive season alters hepatic CYP expression in largemouth bass (Micropterus salmoides). Aquatic Toxicology (Amsterdam, Netherlands), 81: 27- 35.

Brian, W. R., M. A. Sari, M. Iwasaki, T. Shimada, L. S. Kaminsky and F. P. Guengerich (1990). Catalytic activities of human liver cytochrome P- 450 IIIA4 expressed in Saccharomyces cerevisiae. Biochemistry, 29: 11280-11292.

Guengerich, F. P. (2007). Cytochrome p450 and chemical toxicology. Chemical Research in Toxicology, 21: 70-83.

Hannemann, F., A. Bichet, K. M. Ewen and R. Bernhardt (2007). Cytochrome P450 systems-biological variations of electron transport chains. Biochimica et Biophysica Acta (BBA)-General Subjects, 1770: 330-344.

Librado, P. and J. Rozas (2009). DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25: 1451- 1452.

Lynch, T. and A. Price (2007). The effect of cytochrome P450 metabolism on drug response, interactions, and adverse effects. Am. Fam. Physician, 76: 391-396.

Miranda, C. L., M. C. Henderson and D. R. Buhler (1998). Evaluation of chemicals as inhibitors of trout cytochrome P450s. Toxicology and Applied Pharmacology, 148: 237- 244.

Miranda, C. L., J. L. Wang, M. C. Henderson and D. R. Buhler (1989). Purification and characterization of hepatic steroid hydroxylases from untreated rainbow trout. Archives of Biochemistry and Biophysics, 268: 227-238.

Nelson, D. R. (2013). A world of cytochrome P450s. Philosophical Transactions of The Royal Society, B: 368.

Thibaut, R., L. Debrauwer, E. Perdu, A. Goksøyr, J. P. Cravedi and A. Arukwe (2002). Regio-specific hydroxylation of nonylphenol and the involvement of CYP2K-and CYP2M-like iso-enzymes in Atlantic salmon (Salmo salar). Aquatic Toxicology, 56: 177-190.

Zhang, J., J. Yao, R. Wang, Y. Zhang, S. Liu, L. Sun and Z. Liu (2014). The cytochrome P450 genes of channel catfish: their involvement in disease defense responses as revealed by meta-analysis of RNA-Seq datasets. Biochimica et Biophysica Acta, 1840: 2813-2828.

Downloads

Published

2018-01-12

Issue

Section

Articles

Most read articles by the same author(s)

<< < 1 2 3 > >>