Morpho-agronomic and chemical analysis as well as RAPD markers were used to determine the genetic diversity among three Egyptian genotypes of sweet potato; Abees, Mabrouka and Gendawy. The results revealed that there is a wide variation among the three genotypes in most morphological and agronomic characters in addition to the nutritional values. Gendawy genotype had the highest values for most agronomic and chemical traits compared to the other two genotypes; therefore it is considered a good source of agronomic and nutritional traits for breeding. Regarding molecular characterization, a total of nine RAPD primers were used to assess the genetic variability and relationships among the three sweet potato genotypes. A total of 146 amplified bands were generated from the nine primers with 52.74% polymorphism indicating high genetic variability. Cluster analysis revealed a close genetic relationship between Abees and Gendawy genotypes (similarity value of 0.718), while Mabrouka was the most distinct genotype. Results concluded that RAPD analysis could not be effective in separating genotypes according to their morphological, agronomic or chemical characters. In addition, characterization based on these conventional characters should be complemented with DNA-based molecular characterization to reveal genetic diversity in the three Egyptian sweet potato genotypes.

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A.O.A.C. (2005). Official Methods of Analysis, 16th Edition, Association of Official Analytical Chemists, Washington DC. Bovell-Benjamin, A. (2007). Sweet potato: a review of its past, present and future role in human nutrition. Advances in Food and Nutrition Research, 52: 1-59.

da Silva, A. V. C., L. N. T. Andrade, A. R. C. Rabbani, M. U. C. Nunes and L. R. Pinheiro (2014). Genetic diversity of sweet potatoes collection from North-eastern Brazil. Afr. J. Biotechnol., 13: 1109-1116.

Das, A. B. and S. K. Naskar (2008). Genetic variation of high yielding drought resistant sweet potato as evident by RAPD markers. Biological Diversity and Conservation, 1: 28-39.

Elameen, A., S. Fjellheim, A. Larsen, O. A. Rognli, L. Sundheim, S. Msollaand and S. S. Klemsdal (2008). Analysis of genetic diversity in a sweet potato (Ipomoea batatas L.) germplasm collection from Tanzania as revealed by AFLP. Genet. Resour. Crop Evol., 55: 397-408.

Elameen, A., A. Larsen, S. S. Klemsdal, S. Fjellheim, L. Sundheim, S. Msolla, E. Masumba and O. A. Rognli (2011). Phenotypic diversity of plant morphological and root descriptor traits within a sweet potato, Ipomoea batatas (L.) Lam., germplasm collection from Tanzania. Genet. Resour. Crop Evol., 58: 397-407.

FAO (2014). Food and Agriculture Organization of the United Nations. FAOSTAT database. Gepts, P. (2006). Plant genetic resources conservation and utilization: the accomplishments and future of a societal insurance policy. Crop Sci., 46: 2278-2292.

Gichuki, S. T., M. Berenyi, D. Zhang, M. Hermann, J. Schmidt, J. Glössl and K. Burg (2003). Genetic diversity in sweetpotato [Ipomoea batatas (L.) Lam.] in relationship to geographic sources as assessed with RAPD markers. Genet. Resour. Crop Evol., 50: 429-437.

He, G., C. S. Prakash and R. L. Jarret. (1995). Analysis of genetic diversity in a sweetpotato (Ipomoea batatas) germplasm collection using DNA amplification fingerprinting. Genome, 38: 938-945.

He, X. Q., Q. C. Liu, K. Ishiki, H. Zhai, and Y. P. Wang. (2006). Genetic diversity and genetic relationships among Chinese sweetpotato landraces revealed by RAPD and AFLP markers. Breed. Sci., 56: 201-207.

Huang, J. C. and M. Sun (2000). Genetic diversity and relationships of sweetpotato and its wild relatives of the Ipomoea series Batatas (Convolvulaceae) revealed by inter- simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA. Theor. Appl. Genet., 100: 1050-1060.

Jaccard, P. (1901). Étude comparative de la distribuition florale dans une portion des Alpes et des Jura. Bull. Soc. Vandoise Sci. Nat., 37: 547-579.

Khoury, C., B. Laliberté, L. Guarino (2010). Trends in ex situ conservation of plant genetic resources: a review of global crop and regional conservation strategies. Genet. Resour. Crop Evol., 57: 625-639.

Laurie, S. M., F. J. Calitz, P. O. Adebola and A. Lezar (2013). Characterization and evaluation of South African sweet potato (Ipomoea batatas (L.) Lam) landraces. S. Afr. J. Bot., 85: 10-16.

Lin, K. H., Y. C. Lai, H. C. Li, S. F. Lo, L. F. O. Chen and H. F. Lo (2009). Genetic variation and its relationship to root weight in the sweet potato as revealed by RAPD analysis. Sci. Hortic., 120: 2-7.

Luo, K., L. Hui-xiang, W. Zheng-dan, W. Xue-li, Y. Wang, T. Dao-bin, W. Ji-chun and Z. Kai (2016). Genetic diversity and population structure analysis of main sweet potato breeding parents in Southwest China. Scientia Agricultura Sinica, 49: 593-608

Manrique, K. and M. Hermann (2000). Effect of G x E interaction on root yield and betacarotene content of selected sweetpotato (Ipomoea batatas (L.) Lam.) varieties and breeding clones. CIP Program Report 1999-2000, p: 281-287.

Maquia, I., I. Muocha, A. Naico, N. Martins, M. Gouveia, I. Andrade, L. F. Goulao and A. I. Ribeiro (2013). Molecular, morphological and agronomic characterization of the sweet potato (Ipomoea batatas L.) germplasm collection from Mozambique: Genotype selection for drought prone regions. S. Afr. J. Bot., 88: 142-151.

Mohamed, Amina A., Mervat M. M. El-Far and M. E. Saad (2016). Fingerprinting of sweetpotato germplasm using AFLP, RAPD, and SAMPL analysis. Egypt. J. Genet. Cytol., 45: 383-401.

Moulin, M. M., R. Rodrigues, L. S. A. Gonçalves, C. P. Sudré and M. G. Pereira (2012). A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.). Acta Sci. Agron., 34: 139-147.

Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA, 70: 3321-3323.

Saghai-Maroof, M. A., K. M. Soliman, R. A. Jorgensen and R. W. Allard (1984). Ribosomal DNA spacer- length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA, 81: 8014-8018.

Sanoussi, A. F., A. Dansi, H. Ahissou, A. Adebowale, L. O. Sanni, A. Orobiyi, M. Dansi, P. Azokpota and A. Sanni (2016). Possibilities of sweet potato [Ipomoea batatas (L.) Lam] value chain upgrading as revealed by physico-chemical composition of ten elites landraces of Benin. Afr. J. Biotechnol., 15: 481-489.

Scott, G. J., M. W. Rosegrant and C. Ringler (2000). Roots and tubers for the 21st century: trends, projections and policy options. Food, Agriculture and the Environment Discussion Paper No. 31. Washington, DC: International Food Policy Research Institute.

Senanayake, S. A., K. K. D. S. Ranaweera, A. Gunaratne and A. Bamunuarachchi (2013). Comparative analysis of nutritional quality of five different cultivars of sweet potatoes (Ipomea batatas (L) Lam) in Sri Lanka. Food Sci. Nutr., 1: 284-291.

Tairo, F., E. Mneney and A. Kullaya (2008). Morphological and agronomical characterization of sweet potato [Ipomoea batatas (L.) Lam.] germplasm collection from Tanzania. Afr. J. Plant Sci., 2: 77-85.

Tseng, Y. T., H. F. Lo and S. Y. Hwang (2002). Genotyping and assessment of genetic relationships in elite polycross breeding cultivars of sweet potato in Taiwan based on SAMPL polymorphisms. Bot. Bull. Acad. Sin., 43: 99-105.

Veasey, E. A., J. R. Q. Silva, M. S. Rosa, A. Borges, E. A. Bressan and N. Peroni (2007). Phenology and morphological diversity of sweet potato (Ipomoea batatas) landraces of the Vale do Ribeira. Scientia Agricola, 6: 416-427.

Woolfe, J. A. (1992). Sweet potato-an Untapped Food Resource. Cambridge University Press (Published in Collaboration with the International Potato Center, Lima, Peru). Cambridge, UK.

Yada, B., P. Tukamuhabwa, A. Alajo and R. O. M. Mwanga (2010). Morphological characterization of Ugandan sweetpotato germplasm. Crop Sci., 50: 2364-2371.

Zhang, D., M. Ghislain, Z. Huamán, A. Golmirzaie and R. Hijmans (1998). RAPD variation in sweetpotato (Ipomoea batatas (L.) Lam) cultivars from South America and Papua New Guinea. Genet. Resour. Crop Evol., 45: 271-277.

Zhang, D. P., D. Carbajulca, L. Ojeda, G. Rossel, S. Milla, C. Herrera and M. Ghislain (1999). Microsatellite analysis of genetic diversity in sweetpotato varieties from Latin America. CIP Program Report, 2000: 295-301.


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