ASSESSMENT OF GENETIC DIVERSITY IN SOME WILD PLANTS OF ASTERACEAE FAMILY BY RIBOSOMAL DNA SEQUENCE
Abstract
Ribosomal DNA genes are organized in clusters of tandem repeated units, each of which consists of coding regions (18S, 5.8S and 28S) and two internal transcribed spacers (ITS), in addition to intergenic spacer (IGS) region. Accordingly this article is focused on clarifying the sequence divergence of complete rDNA of ITS and IGS regions among four wild and endemic species of Asteraceae family in Egypt. Results indicated that there were a total of 754 and 667 positions across the final dataset for ITS and IGS sequences, respectively. IGS regions were superior compared to ITS region in several parameters like nucleotide diversity rate (π = 0.60), the estimated values of transition/transversion rate ratios (k1 = 38.28, purines), (K2 = 12.58, pyrimidines) and the overall transition/transversion bias (R = 12.10), respectively. This reflects that transitions are more dominant than transversion in Asteraceae germplasm across IGS markers. Thus, it was concluded that the IGS region could be more suitable for measuring genetic relationship in different subspecies of Asteraceae, with more informative sites than ITS sequences. Generally ribosomal DNA particularly intergenic spacer of the rDNA cluster evolves quickly and is highly polymorphic, providing a useful tool for assessing genetic diversity, taxonomic and phylogenetic studies in Asteraceae germplasm.References
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