PHOSPHATE SOLUBILIZING AND BIOCONTROL POTENTIAL OF AN Aspergillus niger ISOLATE FROM EGYPTIAN SOIL
Abstract
Phosphorus (P) is one of the most important plant nutrients that greatly affect overall plant growth. P-mineral always presents in soil with the insoluble condition. Phosphate compounds generally contain aluminum (Al), iron (Fe), manganese (Mn), and calcium (Ca) which vary according to soil type. Phosphate solubilizing fungi play a major role in rising soil phosphate bioavailability for plants by realizing phosphate from its compounds. The present study was aimed to isolate and characterize phosphate solubilizing fungi from Egyptian soil using a solid Pikovskaya (PVK) medium. In total, 6 fungal isolates were able to grow on Pikovskaya (PVK) medium but only one isolate (AG-A01) showed phosphate- solubilizing capacity. DNA was extracted from the isolate followed by amplification of selected Internal Transcribed Spacer (ITS) region and DNA sequencing. After analyzing the DNA sequence, the isolate AG-A01 was identified as Aspergillus niger. The fungal isolate also displayed an antagonism effect against two plant pathogens (Fusarium solani and Fusarium verticillioides). Thus, we consider this fungal isolate as a promising tool for the development of an efficient bio-fertilizer for the plant which has the potential to protect plants from pathogens especially fusarium species.
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