INITIAL CLUSTERING OF SOME EGYPTIAN NEMATICIDES Serratia ISOLATES BASED ON THEIR 16SrRNAs GENE SEQUENCES

Authors

  • SAWSAN Y. ELATEEK Department of Genetics, Faculty of Agriculture, Ain Shams Center of Genetic Engineering and Bio- technology (ACGEB), Ain Shams University, Cairo
  • A. B. ABDEL-RAZIK Department of Genetics, Faculty of Agriculture, Ain Shams Center of Genetic Engineering and Bio- technology (ACGEB), Ain Shams University, Cairo
  • S. A. IBRAHIM Department of Genetics, Faculty of Agriculture, Ain Shams Center of Genetic Engineering and Bio- technology (ACGEB), Ain Shams University, Cairo

Abstract

Biocontrol is known to be the safest agriculture practice for pest control in comparison with pesticides. In addition, pests can be very detrimental to crops, destroying them within a few days. One of the pests the farmers have to deal with on a regular basis is nematodes, which can be extremely destructive to seedlings and whole plants. In our study we shed some light on promising bacterial isolates found to belong to Genus Serratia which can be a good candidate for nematode biocontrol. Eight Serratia isolates were collected from tomato-planted Egyptian soil. They were chosen for this study based on their nematicidal effect. DNA isolation, PCR amplification and partial sequence analysis of 16SrRNA gene were performed. They were aligned using ClustalW with similar strains from the NCBI GenBank database, compared with reference strain of Bacillus thuringiensis JN 315886.1 (as a tree root). BLASTn Microbial genome database, based on their 16SrRNA partial gene sequence, were used to identify the isolates to be mostly correlated to Genus Serratia. Phylogenetic analyses were conducted by MEGA7 using neighbor-joining method with 1000 replicates for bootstrap analysis which indicates 99.4-99.8% similarities with the Serratia nematodiphila and Serratia marcescens species.

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Published

2020-08-30

Issue

Vol. 49 No. 1 (2020)

Section

Articles