MOLECULAR FINGERPRINTING AND MARKER-ASSISTED SELECTION IN CITRUS SPECIES

Authors

  • FATTHY M. ABDEL-TAWAB Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo
  • M A. RASHED Dept. of Genetics, Fac. of Agriculture, Ain Shams Univ., Shoubra El-Kheima, Cairo,Egypt
  • S. A. AZER National Center for Radiation Research and Technology, Nasr City, Cairo, Egypt
  • G. M. I. ELMETABTEB National Center for Radiation Research and Technology, Nasr City, Cairo, Egypt
  • A. F. KHAFAGA National Center for Radiation Research and Technology, Nasr City, Cairo, Egypt

Abstract

Nineteen citrus cultivars were collected; their fingerprint and molecular markers were developed to assist selection for these cultivars. In this study, RAPD was used for the identification of markers associated with Citrus taxa genotypes using 18 arbitrary 10-mer primers. Primers OPA19, OP-C09, OP-D15, OP-G17, OP-L13 and OP-L16 showed monomorphic fragments with no detected polymorphism. The results of primers OP-B07, OP-B11, OP-12, OP-C10, OP-C13 and OP-L20 gave polymorphic fragments with different molecular sizes. No taxa- specific markers were observed for these primers. Primers OP-C15, OP-D01, OP-D07, OPF06, OP-L12 and OP-Z03 showed taxaspecific markers.
PCR reaction was conducted using five ISSR primers. Only two primers HB14 and HA98 showed 100% polymorphic differences among the cultivars, while three primers exhibited high polymorphism such as HB12 (94%) and HA99 (93%).
Two types of pairs of restriction enzymes, Mse1 and PstI, MseI and EcoRI, were used to digest the genomic DNA which was used for AFLP analysis. The three Citurs taxa were separated into two clusters; the first cluster included Balady mandarine and Lime, while the second cluster included Balady orange in all four AFLP combinations.

References

Besnard, G., P. Baradat and A. Bervill (2001). Genetic relationships in the olive (Olea europaea L.) reflect multilocal selection of taxa.Theor. Appl. Genet., 102: 251-258.

Botstein, D., R. L. White, M. Skolnick and R. W. Davis (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Amer. J. Genet., 32: 314-331.

Cabrita, P. Elisiario, J. Leitao, A. Guerreiro, C. Doré, F. Dosba and C. Baril (2001). Assessment of the genetic relationships among Citrus species and varieties by isozyme and RAPD markers. Theor. Appl. Genet., 101: 860-864.

Chaparro, J. X., R. E. Durham, G. A. Moore and W. B. Sherman (1994). Use of isozyme techniques to identify peach X 'Nonpareil' almond hybrids. Hort. Sci., 22: 300-302

Fang, D. Q. and Roose M. L. (1997). Identification of closely related Citrus taxa by inter simple sequence repeats. Theor. Appl. Genet., 95: 408-417.

Fang, D. Q., C.T. Federici, and M. L. Roose (1997). Development of molecular markers linked to a gene controlling fruit acidity in Citrus. Genome, 40: 841-849.

Fang, D., R. R. Krueger and M. L. Roose (1998). Phylogenetic relationships among selected Citrus germplasm accessions revealed by inter simple sequence repeat (ISSR) markers. J. Amer. Soc. Hort. Sci., l23: 612- 617.

Hassan, H. Z., N. M. Mansour and E. A. Abd El-Hady (2002). Molecular characterization of some selected local peach (Prunus persica L.) cultivars in Dakhalia Governorate. Egyp. J. Biotechnology, 12: 68-72.

Koller, B., A. Lehmann, J. M. McDermott and C. Gessler (1993). Identification of apple taxa using RAPD markers. Theor. Appl. Genet., 85: 901-904.

Litt, M. and J. A. Lutty (1989). A hypervariable microsatellite revealed by in vitro amplification of nucleotide repeat within the cardiac muscle actin gene. Amer. J. Hum. Genet., 44: 397-401.

Pasquale, M., R. Garcia, M. J. Asins, J. Forner, E. A. Carbonell (2006). Genetic analysis of apomixes in Citrus and Poncirus by molecular markers. Theor. Appl. Genet., 99: 511-518.

Sanker, A. A. and G. A. Moore (2001). Evaluation of inter simple sequence repeat analysis for mapping in Citrus and extension of the genetic linkage map. Theor. Appl. Genet., 102: 206-214

Stegmann, H., A. M. R. Affify and K. R. F. Hussein (1985). Cultivar identification of dates (Phoenix dectylifera) by protein patterns. 2nd International Symposium of Biochemical Approaches to identification of taxa. Braunschweig, West Germany, pp 44.

Testolin, R. W. G. Huang, O. Lain, R. Messina, A. Vecchione and G. Cipriani (2001). A kiwifruit (Actinidia spp.) linkage map based on microsatellites and integrated with AFLP markers. Theor. Appl. Genet., 103: 30-36.

Vos, P., R. Hogers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman and M. Kuiper (1995). AFLP: a new technique for DNA fingerprinting. Nucl. Acids Res., 23: 4407-4414.

Wang, Y., L. L. Georgi, G. L. Reighard, R. Scorza and A. Abbott (2002). Genetic mapping of the evergrowing gene in peach [Prunus persica (L.) Batsch.]. The American Gene Assoc., 93: 352-358.

Warburton, M. L. V. L. Becerra- Velasquez, J. C. Goffreda and F. A. Bliss (1996). Utility of RAPD markers in identifying genetic linkages to genes of economic interest in peach. Theor. Appl. Genet., 93: 920-925.

Williams, J. G. K., A. R. Kublik, K. J. Livak, J. A. Rafalsky and S. V. Tingey (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res., 18: 6531-6535.

Zabeau, M. and P. Vos (1993). Selective restriction fragment amplification, a general method for DNA fingerprinting. Nucl. Acids Res., 18: 7213-7218.

Downloads

Published

2016-01-13

Issue

Vol. 37 No. 2 (2008)

Section

Articles

Most read articles by the same author(s)