GROWTH, YIELD PARAMETERS AND GENETIC DIVERSITY AMONG SOME EGYPTIAN FABA BEAN (Vicia faba L.) CULTIVARS USING SCOT AND ITS MARKERS ANALYSES
Abstract
Evaluation of growth, yield and quality parameters as well as, molecular variation among seven Egyptian faba bean (Nubaria 1, 2, 5, Sakha 4, Giza 716, Giza 843 and Wady 1 ) cultivars using ITS and SCoT molecular markers were conducted in this present investigations. Field experiments confirmed highly significant diversity among all tested faba bean cultivars in all tested parameters during the two tested seasons. The highest emergence percentages were recorded in Giza 843 cultivar. The obtained results showed that while Giza 716 cultivar was superior to all other tested cultivars in almost growth and yield traits during both seasons. Nubaria 1 had the highest values of seed total protein and carbohydrates content in both seasons. Molecular studies with seven SCoT primers showed that all studied cultivars exhibited 100% polymorphism. SCoT 1 and SCoT 2 produced 6 and 5 polymorphic bands, respectively, while SCoT 4 yielded 4 polymorphic bands with 2 unique bands. The study also utilized Principal Component Analysis (PCA), which explained 66.6% of the total genetic variation, showing close clustering of Nubaria 2, Nubaria 5, Giza 716, and Giza 843, while Wady 1 displayed clear genetic divergence. ITS-based phylogenetic analysis clustered Nubaria 1 and Nubaria 2 closely, while Sakha 4, Giza 843, and Wady 1 exhibited distinct genetic traits. Finally, this investigation showed that under both agronomical and molecular levels, there were a clear variation among almost of studied faba bean cultivars, which might be provide good material for breeding programs and genetic improvement of faba beans.
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