GENETIC DIVERSITY OF TWO PHENOTYPES OF EGYPTIAN DOMESTIC GEESE Anser anser (AVES: ANATIDAE) BASED ON SSR MARKERS
Abstract
Genetic variation within and among domestic whitish grey and white Greylag geese populations collected from Minia, Upper Egypt were examined using ten SSR microsatellite primers. Of these primers, only eight primers produced scorable amplified bands, while TTUCG-1 and APH17 primers generated no ampli-fication products in any of studied geese population. These scorable amplified bands displayed variable polymorphism amongst the whitish grey and white popu-lations. The total number of amplified bands produced by eight primers was 29 bands with an overall mean (3.63 ± 0.89) and ranged from one band with TTUCG-2 and TTUCG-4 primers to eight bands with SFIMU-1 primer. Primers APH11, APH21 and SFIMU-1 revealed 100% polymorphism between geese popula-tions. Cluster analysis of similarity within populations indicated: 1) the existence of two distinct evolutionary clusters corre-sponding to whitish grey and white geese groups and 2) clustering of white popula-tions with the whitish grey population. The average similarity among the two geese phenotypes was nearly similar; this suggests their relatedness to each other. This relationship between the two geese phenotypes strongly supports the previous hypothesis of including the white geese within the genus Anser basically contain-ing the Greylag geese. It is the first time to use SSR markers for identification of Egyptian domestic geese breed.References
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