BREAD WHEAT COMPARATIVE EXPRESSION ANALYSIS IN RESPONSE TO LEAF RUST (puccinia triticina) USING MICROAR- RAY TECHNIQUES
Abstract
Wheat is one of the major food crops in the world and a foundation of human nutrition worldwide. In addition to its basic caloric value, wheat with its high protein content is one of the most important sources of plant protein in the human diet. Egypt faces many challenges in wheat production and pest management. Besides, there is increasing concern over environmental issues. These issues will continue to be important into the future. Two local bread wheat Cultivars (Misr1 and Gemmiza7) against leaf rust (Puccinia triticinae) were examined. About 18203 genes were differentially expressed in response to leaf rust treatment in the cultivar Misr1 and 11303 genes in Gemmeiza7 cultivar. Within the differentially expressed genes, 6589 genes were differentially expressed in both cultivars which were vary in their behavior according to the cultivar. Misr1 showed 3460 up-regulated genes and 3129 down-regulated genes, while Gemmiza7 had 3249 up-regulated genes and 3340 down-regulated genes. We also discovered 486 genes with opposite expression between the two studied cultivars. The current study resulted in a huge pool of genes associated with resistance to biological stresses and fungal resistant, which needs more studies to draw an integrated picture of genetic interference that leads to resistance to biological stresses. The current study also suggested the need to study the time component as an influencing factor in the resistance of biological stresses.
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