FUNCTIONAL GENOMIC PROFILING OF DROUGHT RESPONSIVE Micro-RNA IN WHEAT
Abstract
MicroRNAs are small non-coding RNAs that play a major regulatory role in post translation regulation either by translation inhibition or mRNA cleavage. Recent studies proved that miRNAs have a regulatory role in both abiotic and biotic stress in plants. In our study, we measure the expression profile of 10 known abiotic stress miRNAs in hexaploid bread wheat (Triticum aestivum L.), under drought stress in the seedling stage. Significant upregulation was observed with osa_miR319a-3p.2-3, miR5048, gmamiR5783, hci-miR156a, bdi-miR159b-3p, zma-miR164g-3, ssl-miR398 and ptcmiR482c- 5p. Among these, hci-miR156a, and osa-miR319a-3p.2-3 showed downregulation after 12 h compared to 2 h treatment. Only osa-miR172b was downregulated. This variant and dynamic expression patterns is good evidence and certain indicator of miRNA correlation to drought tolerance mechanisms present in wheat. This is the first data that provides accurate measurement of some drought related miRNA in wheat in the early exposure to drought stress and in the identification of several genes that could be implicated in drought tolerance and could be used for improving wheat and other cereal crops in this respect.References
Beauclair, L., A. Yu and N. Bouche (2010). Micro-RNA directed cleavage and translational repression of the copper chaperone for superoxide dismutase mRNA in Arabidopsis. Plant J., 62: 454- e462.
Dai, X., and P. X. Zhao (2011). psRNATarget: a plant small RNA target analysis server. Nucleic Acid. Res., 39: W155eW159. http://dx.doi.org/10.1093/nar/gkr31 9.
Eldem, V., S. Okay and T. Unver (2013). Plant microRNAs: new players in functional genomics. Turk. J. Agric., 37: 1-21
Gupta, O. P., N. L. Meena, I. Sharma and P. Sharma (2014). Differential regulation of microRNAs in response to osmotic, salt and cold stresses in wheat. Mol. Biol. Rep., 41: 4623- 4629.
Inal, B., M. Turktas, H. Eren, E. Ilhan, S. Okay, M. Atak, M. Erayman and T. Unver (2014). Genome-wide fungal stress responsive miRNA expression in wheat. Planta, 240: 1287-1298.
Jin, J., H. Zhang, L. Kong, G. Gao and J. Luo (2014). Plant TFDB 3.0: a portal for the functional and evolutionary study of plant transcription factors. Nucleic Acid. Res., 42: D1182eD1187. http://dx.doi.org/10.1093/nar/gkt10 16.
Kantar, M., T. Unver and H. Budak (2010). Regulation of barley miRNAs upon dehydration stress correlated with target gene expression. Funct. Integr. Genome, 10: 493-507.
Liu, H. H., X. Tian, Y. J. Li, C. A. Wu and C. C. Zheng (2008). Microarray- based analysis of stressregulated microRNAs in Arabidopsis thaliana. RNA 14: 836e843. http:// dx.doi.org/10.1261/rna. 895308.
Mittal, D., S. K. Mukherjee, M. Vasudevan and N. S. Mishra (2013). Identification of tissuepreferential expression patterns of rice miRNAs. J. Cell. Biochem., 114: 2071e2081. http://dx.doi. org/10.1002/jcb.2455.
Pandey, R., G. Joshi, A. R. Bhardwaj, M. Agarwal and S. Katiyar-Agarwal (2014). A comprehensive genomewide study on tissue-specific and abiotic stress-specific miRNAs in Triticum aestivum. PLoS One 9: e95800.
Sunkar, R., V. Chinnusamy, J. Zhu and J. K. Zhu (2007). Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. Trends Plant Sci., 12: 301-309.
Trindade, I., C. Capitao, T. Dalmay, M. P. Fevereiro and D. M. Santos (2010). miR398 and miR408 are upregulated in response to water deficit in Medicago truncatula. Planta, 231: 705-716.
Unver, T., D. M. Namuth-Covert and H. Budak (2009). Review of current methodological approaches for characterizing microRNAs in plants. Int. J. Plant Genomics, 2009: 262463. doi:10.1155/2009/ 262463.
Xin, M., Y. Wang, Y. Yao, C. Xie, H. Peng, Z. Ni and Q. Sun (2010). Diverse set of microRNAs are responsive to powdery mildew infection and heat stress in wheat (Triticum aestivum L.). BMC. Plant Biol., 10: 123. doi: 10.1186/1471- 2229-10-123.
Zhang, B. (2015). MicroRNA: a new target for improving plant tolerance to abiotic stress. J. Exp. Bot., 66: 1749-1761. doi:10.1093/jxb/erv013.
Zhao, B., R. Liang, L. Ge, W. Li, H. Xiao, H. Lin, K. Ruan and Y. Jin (2007). Identification of drought-induced microRNAs in rice. Biochem. Biophys. Res. Commun., 354: 585- 590.
Zhu, H., F. Hu, R. Wang, X. Zhou, S. H. Sze, W. Liou Lisa, A. Barefoot, M. Dickman and X. Zhang (2011). Arabidopsis Argonaute10 specifically Sequesters miR166/165 to regulate shoot apical meristem development. Cell, 145: 242e256.
