SINGLE - STRAND CONFORMATION POLYMORPHISM OF MYOSTATIN GENE LINKED TO SLAUGHTER TRAITS AND SOME BLOOD CONSTITUENTS IN RABBITS
Abstract
Myostatin (MSTN) gene expressed mainly in muscles of animals and human. It acts as a negative regulator of skeletal muscle development that limits skeletal muscle mass. Several mutations have been identified in the MSTN gene which natu-rally occurred in this gene leading to in-crease in skeletal muscle mass conse-quently better growth performance for animal. It has been considered as a candi-date gene for meat selection programs in domestic animals. Therefore, this study aimed to use Polymerase Chain Reaction Single-Strand Conformation Polymor-phism (PCR-SSCP) method to explore genetic polymorphisms of the part of in-tron 2, the coding sequence of exon 3 and a part of the 3’ untranslated region of MSTN gene in rabbits. Also the study evaluated genetic polymorphism of the MSTN gene on slaughter traits and some blood constituents as plasma total protein, Albumin, Globulin, serum Ca and IP in addition to the effect of line and sex on the above traits was also studied in the present investigation. So, twenty four rabbits (12♀ and 12♂) at 9 weeks of age (Slaugh-ter age) from each V and Alex lines were selected for genotyping this segment of rabbit MSTN gene. The analysis of PCR-SSCP revealed two SSCP patterns i.e., AB and AA in V population with a genotypic frequency of 0.92 and 0.08, respectively. While, Alex population showed one type of pattern i.e. AB with a frequency of 1.00 in the MSTN gene. The two SSCP geno-types had two alleles i.e. A and B alleles. The frequency of A allele was 0.54, 0.50, while the B allele showed a frequency of 0.46 and 0.50, respectively in V and Alex populations. MSTN locus was highly in-formative and showed high heterozygosity in both lines. AB genotype always record-ed higher score in carcass%, dressing%, liver % and carcass length than AA geno-type. Plasma glucose concentration was affected by MSTN polymorphisms that revealed significant differences with AA compared with AB genotype. Alex line had significant influence on abdominal fat percentage. Also, sex had significant ef-fect on total protein and globulin (g/d) as males showed significant increase in these traits than female's rabbits.References
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