ISOLATION AND IDENTIFICATION OF LECTIN GENE IN LICO- RICE, Glycorrhiza glabra L., PLANT IN EGYPT
Abstract
Lectins are a group of nonenzymatic carbohydrate-binding proteins that are present in plants, animals and microorganisms. In the present study, DNA was extracted from the dried roots of licorice and seeds of green pea plants (positive control). Specific PCR technique was employed using specific primers (Lec1 and Lec2) for the amplification of Lectin gene. The amplified fragment mol. Size was about 700 bp that was cloned and sub-cloned into the pPROEXHTa expression vector. The purified proteins were separated at 27 and 30 KDa in both licorice and green pea plants, respectively, using SDS-PAGE. Sequencing of the PCR product of lectin gene was documented into the Genbank under the accession numbers HQ337023 and HQ337024 for licorice and green peas plants, respectively. The comparison of nucleotide sequence of the root Lectin gene from Licorice under study showed complete similarity (100%) to partial lectin 2 gene from Licorice that accession number (AJ234389) while, lectin sequence from seeds of green peas was clearly shared 100% nucleotide identity with a lectin (psl), complete cds and lecA genes from green pea with their accession numbers EU825771 and T00440, respectively, also, it showed nucleotide sequence identity of 98%. to the P. sativum psl lectin gene (xx66368) and Pea psl gene encoding Lectin complete cds (M18160) with identity of 92%.References
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