DETECTION OF FIBER QUALITY GENES IN SOME PLANTS BELONGING TO FAMILY Asclepiadaceae
Abstract
Two fiber plant species belonging to the family Asclipidaceae; Calotropis procera and pergularia daemia were chosen for the present study. Gene expression analysis of fiber quality genes (EXPA3 and EXPA4) was achieved using quantitative real-time PCR (qRT-PCR) in two plant parts (leaf and stem) in C. procera showing a high expression level of EXPA3 gene compared to the EXPA4 gene. Moreover, the expression level in stems is higher than that in leaves regardless of plant species. Detection of genes (EXPA3) was carried out by PCR reaction producing band in Calotropis procera with sizes 850bp but for EXPA4 gene PCR product in C. procera and Pergularia daemia with sizes 450bp. The obtained fragments were sequenced and phylogenetic trees based on amino acid sequences using Mega 7.0 software were accomplished. The results revealed the close relatedness of CpEXPA3 understudy to C. procera (ABO30977.1) was recorded in the National Center for Biotechnology Information (NCBI) database. In addition, the close relatedness of CpEXPA4 understudy to CpEXPA4 in C. procera (EF434784.2) was recorded in the NCBI database. The present study recommends conducting more researches on fiber crops due to their economic importance. Furthermore, their genetic potentialities which enable them to be good genetic resources and alternatives to economically important fiber crops. Moreover, For Guidance to domestication and cultivation of inexpensive fiber resources that are tolerant to various environmental stresses and do not exhaust the farmlands.
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