MOLECULAR IDENTIFICATION AND PHYLOGENETIC RELATIONSHIPS OF Origanum syriacum L. AND Origanum vulgare L. US- ING CHLOROPLAST rbcL AND matK BARCODES GENES
Abstract
Genus Origanum is one of the most species-rich as medicinal plants and pharmaceutically in the family Lamiaceae for several multipurpose used, and thus it is an endangered plant that needs a strict conservation plan. This genus contains many plants with medical uses, and thus an objective identification method is urgently needed. DNA barcoding is a sample fast technique at molecular level in the field of identification, authentication, classification and differentiation between two Origanum species and with other species. The current investigation was conducted to identify, discriminate and authenticate Origanum vulgare L. and Origanum syriacum L. subsp. sinaicum using two chloroplast genes (coding sequences) as the most common DNA barcodes, ribulose 1, 5-biphosphate carboxylase large subunit (rbcL) and maturase K (matK) genes. The partial sequence length of rbcL gene of two Origanum species were 528bp and Lamiaceae and take them away from five plant species from different families as out group of the family Lamiaceae. The obtained results revealed that rbcL and matK genes nucleotide sequence isolated from the two Origanum species in this investi- gation showed high similarities and closely related to NCBI recoded 17 genera be- longing to the family Lamiaceae. Furthermore, a phylogenetic tree analyses were constructed based on amino acid sequence of 60 rbcL and 60 matK genes using MEGA 7 program by Maximum Likelihood (ML) method with the highest log likelihood (-2836.15) for rbcL gene and (-5692.60) for matK gene
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