EFFECT OF AGROTIS SEGETUM NUCLEOPOLYHEDROVIRUS VIRAL ENHANCING FACTOR 1 (Vef 1) ON THE LETHALITY OF AUTOGRAPHA CALIFORNICA MULTIPLE NUCLEOPOLYHEDROVIRUS AGAINST EGYPTIAN COTTON LEAFWORM (Spodoptera littoralis)
Abstract
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) cannot infect the Egyptian cotton leafworm, Spodoptera littoralis larvae per os. How-ever, successful infection can be observed via larvae haemocoel injection using AcMNPV BVs. Baculovirus enhancins, known also as viral enhancing factors (VEFs), have shown to enhance infectivi-ty of some baculoviruses toward non-natural hosts. In the present study, a re-combinant AcMNPV (vAcMNPV-enAg1) that expresses the Viral Enhancing Factor 1 (vef1) originated from an enhancin-rich Polish isolate of Agrotis segetum nucleopolyhedrovirus (AgseNPV-A) was constructed. The vef1 gene was expressed under the control of the p10 promoter in the presence of the polyhedrin gene. The recombinant vAcMNPV-enAg1 was gen-erated to examine the possible enhance-ment of AcMNPV lethality against S. littoralis larvae per os. Using RT-PCR, successful transcription of vef-1 mRNA was detected in a time course intervals, and occlusion bodies production was determined in Sf9 cells infected with vAcMNPV-enAg1. However, no tran-scripts were detected in mRNA isolated from mid-guts of larvae infected using vAcMNPV-enAg1. In addition, the pro-duction of the recombinant virus OBs was 5-fold lower than that of the wild type AcMNPV. Biological analysis of the progeny OBs isolated from injected S. littoralis larvae using the recombinant virus did not show significant improve-ment of virus lethality against S. littoralis neonates per os compared to the wild type AcMNPV. Hence, the results suggested that the failure of S. littoralis infection using AcMNPV per os is more likely unrelated to enhancin activity.References
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