ACTIVATION OF Bare-1 RETROTRANSPOSONS IN BARLEY UNDER SORBITOL STRESS

Authors

  • A. MANSOUR Genetics Department, Faculty of Agriculture, Zagazig University, Egypt
  • M. J. JääSKELäINEN MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki, PO. Box 56, Viikinkaari 9, FIN-00014 Helsinki, Finland
  • W. CHANG MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki, PO. Box 56, Viikinkaari 9, FIN-00014 Helsinki, Finland
  • A. H. SCHULMAN MTT/BI Plant Genomics Laboratory, Institute of Biotechnology, University of Helsinki, PO. Box 56, Viikinkaari 9, FIN-00014 Helsinki, Finland

Abstract

LTR-retrotransposons and other repetitive DNA elements are directly or indirectly responding to a wide variety of stresses by increasing or decreasing its copies. This effect is specific for different retrotransposons or stresses. The Bare-1 retrotransposon members are actively transcribed in vivo in barley. Bare-1 family was reported to respond to sharp microclimatic divergence specially drought. Sorbitol has been used widely to mimic the effects of drought. A potential osmotically-stressed action has been ascribed to sorbitol, but invivo evidence of this remains elusive. In the present work, the effect of sorbitol was compared in both Copia and Gypsy groups of retrotransposon using specific primers for both groups. One step RT-PCR analysis showed that sorbitol exerted a strong influence upon Copia elements group after 4, 24 and 34 hours of sorbitol treatment. When Bare-1 specific primers were used to amplify Copia cDNA products, it revealed unique strong DNA bands at the same time points. The immunobloting of Bare-1 Gag protein specific antibody showed no specific increase after these treatments. Hence, sorbitol, has the capacity, in barley plant, to increase the transcriptional activity of Copia elements specially Bare-1 retrotransposon.

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Published

2016-01-13

Issue

Vol. 37 No. 2 (2008)

Section

Articles