DETECTION OF WIDE COMPATIBILITY AND RESTOEING ABILITY GENES USING MOLECUAR MARKERS IN RICE
Abstract
Explanation of heterosis between Indica and Japonica subspecies of rice (Oryza sativa L.) has been reported, but for a long time, this heterosis has not been directly utilized because of the associated effects of high sterility in F1 and poor filling of spikelets, and obvious transgression of plant height and growth duration. Twenty promising lines were selected from the fourth segregating generations which derived from the cross between Giza178 x Dular (Dular is a donor for WC genes, while Giza178 is a donor for Rf genes). The selection of these F4 lines depends on the morphological marker C+ (chromogene for apiculus color) that linked with WC gene. DNA was extracted from the selected lines and used for screening the WC and Rf genes using SSR markers to confirm the presence of restorer genes as well as wide compatibility genes. The results confirmed that Dular variety has two alleles (S-5 and S-8) for WC, while, Giza178 has three alleles (Rf-1, Rf-3 and Rf-4) for Rf. The selected lines number 2 and 10 have five alleles (S-5, S-8, Rf-1, Rf-3 and Rf-4) for WC and Rf genes. These lines might have WC and Rf genes. Thus, they could be used as widely compatible restorer lines with Indicia and Japonica CMS lines to produce Indicia/Japonica hybrids.References
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